Fernandes Lídia de Oliveira, Mendes Soares Igor Paulino, Anselmi Caroline, Pires Maria Luiza Barucci Araujo, Ribeiro Rafael Antonio de Oliveira, Peruchi Victória, de Souza Costa Carlos Alberto, Hebling Josimeri
Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.
Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.
Clin Oral Investig. 2023 Dec;27(12):7295-7306. doi: 10.1007/s00784-023-05320-8. Epub 2023 Oct 18.
To investigate the response of pulp cells to the application of silver diamine fluoride (SDF) and potassium iodide (KI) on demineralized dentin.
The occlusal surfaces of human dentin discs (0.4 mm thick) with similar permeability were subjected to an artificial caries protocol, and then the discs were adapted into artificial pulp chambers. MDPC-23 cells were seeded on the healthy pulp dentin surface, while the demineralized surface was treated with SDF, KI, SDF + KI, or hydrogen peroxide (positive control-PC) (n = 8). The negative control (NC) received ultrapure water. After 24 h, cell viability (alamarBlue) and morphology (SEM) were evaluated. The extracts were then applied to new MDPC-23 cells seeded in culture plates to assess their viability and the formation of mineralized nodules (MN; Alizarin Red) after seven days. The data were analyzed using one-way analysis of variance/Tukey or Games-Howell tests (α = 5%).
SDF and PC significantly reduced the viability of cells seeded on discs (45.6% and 71.0%, respectively). Only cells treated with SDF or PC detached from the dentin substrate, while the remaining cells showed altered morphology. Cells in contact with extracts showed less reduction in viability, but it was still more toxic compared to NC. Only PC reduced MN deposition. SDF + KI or KI alone did not affect the cell response.
SDF applied alone showed a mild to moderate transdentinal cytotoxic effect on pulp cells. However, the combination of SDF + KI reduced the cytotoxic effects. Both materials used alone or in combination did not affect the mineralization ability of pulp cells.
Besides improving esthetic results, associating potassium iodide with silver diamine fluoride may reduce the transdentinal cytotoxic effects of this cariostatic agent on pulp cells.
研究牙髓细胞对氟化亚锡（SDF）和碘化钾（KI）作用于脱矿牙本质的反应。
将具有相似渗透性的人牙本质片（0.4毫米厚）的咬合面进行人工龋损处理，然后将牙本质片适配到人工牙髓腔中。将MDPC-23细胞接种在健康的牙髓牙本质表面，而脱矿表面分别用SDF、KI、SDF + KI或过氧化氢（阳性对照-PC）处理（n = 8）。阴性对照（NC）接受超纯水。24小时后，评估细胞活力（alamarBlue）和形态（扫描电子显微镜）。然后将提取物应用于接种在培养板中的新MDPC-23细胞，以评估7天后它们的活力和矿化结节（MN；茜素红）的形成。使用单因素方差分析/Tukey或Games-Howell检验（α = 5%）分析数据。
SDF和PC显著降低了接种在牙本质片上的细胞活力（分别为45.6%和71.0%）。仅用SDF或PC处理的细胞从牙本质基质上脱离，而其余细胞显示形态改变。与提取物接触的细胞活力降低较少，但与NC相比仍毒性更大。只有PC减少了MN沉积。SDF + KI或单独的KI不影响细胞反应。
单独应用SDF对牙髓细胞显示出轻度至中度的穿髓细胞毒性作用。然而，SDF + KI的组合降低了细胞毒性作用。单独或联合使用的两种材料均不影响牙髓细胞的矿化能力。
除了改善美学效果外，将碘化钾与氟化亚锡联合使用可能会降低这种防龋剂对牙髓细胞的穿髓细胞毒性作用。
