Cai Chen Yi, Meng Fei Long, Rao Lin, Liu Yun Yue, Zhao Xiao Li
Key Research Laboratory for Cell and Gene Engineering of Zhejiang Provincial, Institute of Genetics and Regenerative Biology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
Yi Chuan. 2020 Nov 20;42(11):1042-1061. doi: 10.16288/j.yczz.20-235.
Since Takahashi and Yamanaka reported the generation of induced pluripotent stem cells (iPSCs) in 2006, the field of pluripotent stem cells has entered an unprecedented state of development. It plays an important role in disease modeling, drug discovery and cell therapy, and promotes the development of cell biology and regenerative medicine. At present, iPSC technology has become an important tool for studying of pathological mechanisms. New drugs screened by iPSC technology are being developed, and the number of clinical trials using iPSC-derived cells is gradually increasing. The latest research progress of iPSCs, combined with gene editing technology and 3D organoid methodology, promotes the further applications of iPSCs in disease research. In this review, we introduce the innovation of reprogramming methods in recent years, analyze the advantages and disadvantages of four reprogramming methods: integrated virus vector system, integrated non-viral vector system, non-integrated virus vector system and non-integrated non virus vector system. At the same time, we summarize the latest research progress on iPSCs in disease modeling and clinical treatment strategies, so as to provide a reference for further in-depth research in various fields of iPSCs.
自2006年高桥和山中报道诱导多能干细胞（iPSC）的产生以来，多能干细胞领域进入了前所未有的发展阶段。它在疾病建模、药物发现和细胞治疗中发挥着重要作用，推动了细胞生物学和再生医学的发展。目前，iPSC技术已成为研究病理机制的重要工具。通过iPSC技术筛选的新药正在研发中，使用iPSC衍生细胞的临床试验数量也在逐渐增加。iPSC的最新研究进展，结合基因编辑技术和3D类器官方法，推动了iPSC在疾病研究中的进一步应用。在本综述中，我们介绍了近年来重编程方法的创新，分析了四种重编程方法的优缺点：整合病毒载体系统、整合非病毒载体系统、非整合病毒载体系统和非整合非病毒载体系统。同时，我们总结了iPSC在疾病建模和临床治疗策略方面的最新研究进展，为iPSC各领域的进一步深入研究提供参考。
