Assiut Health Administration, Assiut, Egypt.
Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, 71515, Egypt.
Sci Rep. 2020 Nov 23;10(1):20383. doi: 10.1038/s41598-020-77416-0.
Type II diabetes (T2D) may worsen the course of hepatitis C virus infection with a greater risk of liver cirrhosis (LC) and hepatocellular carcinoma (HCC). In chronic viral infections, the deranged B cell subset signifies uncontrolled disease. The study aimed to verify the relation between B cell subsets' distribution and liver disease progression in chronic hepatitis C (CHC) patients with T2D. A total of 67 CHC patients were divided into two groups; 33 non-diabetic and 34 with T2D. Each group was subdivided into CHC-without LC or HCC (N-CHC), CHC-with LC (CHC-LC), and CHC-with HCC (CHC-HCC). Twenty-seven healthy individuals also participated as controls. Flow cytometry was used to analyze CD19 B cell subsets based on the expression of CD24 and CD38. CD19CD24CD38 Immature/transitional B cells elevated in diabetic than non-diabetic patients. In diabetic patients, while CD19CD24CD38 primarily memory B cells were higher in CHC-N and CHC-HCC groups than LC with a good predictive accuracy of LC, the opposite was observed for CD19CD24CD38 new memory B cells. Only in diabetic patients, the CD19CD24CD38 naïve mature B cells were high in CHC-HCC patients with good prognostic accuracy of HCC. Merely in diabetic patients, several correlations were observed between B cell subsets and liver function. Immature/transitional B cells increase remarkably in diabetic CHCpatients and might have a role in liver disease progression. Memory and Naïve B cells are good potential predictors of LC and HCCin diabetic CHCpatients, respectively. Further studies are needed to investigate the role of the CD19CD24CD38 new memory B cells in disease progression in CHC patients.
2 型糖尿病（T2D）可能使丙型肝炎病毒（HCV）感染的病程恶化，增加肝硬化（LC）和肝细胞癌（HCC）的风险。在慢性病毒感染中，紊乱的 B 细胞亚群表明疾病失控。本研究旨在验证 T2D 合并 CHC 患者的 B 细胞亚群分布与肝病进展之间的关系。共纳入 67 例 CHC 患者，分为 2 组：33 例非糖尿病和 34 例 T2D。每组再分为无 LC 或 HCC 的 CHC（N-CHC）、LC 的 CHC（CHC-LC）和 HCC 的 CHC（CHC-HCC）。27 例健康个体作为对照。采用流式细胞术根据 CD24 和 CD38 的表达分析 CD19 B 细胞亚群。与非糖尿病患者相比，糖尿病患者的 CD19CD24CD38 幼稚/过渡 B 细胞升高。在糖尿病患者中，虽然 CD19CD24CD38 主要记忆 B 细胞在 CHC-N 和 CHC-HCC 组中高于 LC，对 LC 有较好的预测准确性，但在 CD19CD24CD38 新记忆 B 细胞中则相反。仅在糖尿病患者中，CD19CD24CD38 幼稚成熟 B 细胞在 CHC-HCC 患者中较高，对 HCC 有较好的预后准确性。仅在糖尿病患者中，B 细胞亚群与肝功能之间存在一些相关性。幼稚/过渡 B 细胞在糖尿病 CHC 患者中显著增加，可能在肝病进展中起作用。记忆和幼稚 B 细胞分别是糖尿病 CHC 患者 LC 和 HCC 的良好潜在预测因子。需要进一步研究以探讨 CD19CD24CD38 新记忆 B 细胞在 CHC 患者疾病进展中的作用。
