Wei Lingling, Zhang Lijie, Zhao Dong, Ma Yan, Yang Longyan
Center for Endocrine Metabolism and Immune Diseases, Beijing Luhe Hospital, Capital Medical University, Beijing, 101149, China.
Beijing Key Laboratory of Diabetes Research and Care, Beijing, 101149, China.
Endocrine. 2025 Feb;87(2):562-568. doi: 10.1007/s12020-024-04040-7. Epub 2024 Sep 23.
To investigate the aberrant distribution and clinical relevance of regulatory B cells (Bregs) subsets in the peripheral blood of individuals with different levels of insulin resistance (IR).
A cohort of 124 subjects were divided into five groups according to their insulin resistance index (HOMA-IR) and diabetes diagnosis. The groups comprised Group 1 (IR with good glycemic control) and Group 2 (IR with poor glycemic control) at HOMA-IR < 3, Group 3 (IR without T2DM) and Group 4 (IR with T2DM), at 3 ≤ HOMA-IR < 6, and Group 5 (IR with T2DM) at HOMA-IR ≥ 6. Peripheral blood samples were collected from each group, the percentages of CD19CD24CD27 and CD19CD24CD38 Bregs and the levels of IL-2, IL-4, IL-6, IL-10, IL-17, TNF-α, IFN-γ were detected by flow cytometry and flow microsphere matrix method. Additionally, the cytokines levels were validated through ELISA. The activation of Bregs and the production of IL-10 among different groups were analyzed. Spearman correlation analysis was used to analyze the correlation between Bregs activation rate and IR degree.
The results showed that the levels of CD19CD24CD27 and CD19CD24CD38 cells were increased whether in IR without or with type 2 diabetes mellitus (T2DM) groups compared to the IR groups, with the most significant increase observed in Group 5. Moreover, the plasma levels of IL-6, IL-10, IL-17, TNF-α and IFN-γ in the IR group were higher than those in the IR group. The expression and activation level of Bregs were positively correlated with the severity of IR in T2DM.
These results suggest that the increase level of Bregs is closely related to the severity of IR, highlighting the potential significance of Bregs in the clinical progression of T2DM and its associated insulin resistance.
探讨不同胰岛素抵抗（IR）水平个体外周血中调节性B细胞（Bregs）亚群的异常分布及其临床相关性。
根据胰岛素抵抗指数（HOMA-IR）和糖尿病诊断情况，将124名受试者分为五组。HOMA-IR＜3时，分为1组（血糖控制良好的IR）和2组（血糖控制不佳的IR）；3≤HOMA-IR＜6时，分为3组（无2型糖尿病的IR）和4组（有2型糖尿病的IR）；HOMA-IR≥6时为5组（有2型糖尿病的IR）。采集每组外周血样本，采用流式细胞术和流式微球基质法检测CD19CD24CD27和CD19CD24CD38 Bregs的百分比以及IL-2、IL-4、IL-6、IL-10、IL-17、TNF-α、IFN-γ的水平。此外，通过酶联免疫吸附测定法（ELISA）验证细胞因子水平。分析不同组中Bregs的活化情况以及IL-10的产生。采用Spearman相关性分析来分析Bregs活化率与IR程度之间的相关性。
结果显示，与IR组相比，无论有无2型糖尿病（T2DM）的IR组中CD19CD24CD27和CD19CD24CD38细胞水平均升高，其中5组升高最为显著。此外，IR组中IL-6、IL-10、IL-17、TNF-α和IFN-γ的血浆水平高于IR组。T2DM中Bregs的表达和活化水平与IR的严重程度呈正相关。
这些结果表明，Bregs水平的升高与IR的严重程度密切相关，突出了Bregs在T2DM临床进展及其相关胰岛素抵抗中的潜在意义。
