Lei Caixia, Zhang Shuo, Zhu Saijuan, Wu Junping, Xiao Min, Zhou Jing, Fu Jing, Sun Yijuan, Xu Congjian, Sun Xiaoxi
Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, 200011, China.
Key Laboratory of Female Reproductive Endocrine Related Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, 200011, China.
J Assist Reprod Genet. 2021 Jan;38(1):129-138. doi: 10.1007/s10815-020-02013-z. Epub 2020 Nov 23.
To evaluate whether the morphologically normal spermatozoa selected for intracytoplasmic sperm injection (ICSI) under microscope had a higher rate of normal/balanced chromosome contents than that in the whole unselected sperm from reciprocal translocation carriers.
Five hundred unselected spermatozoa from each of 40 male translocation carriers were performed with fluorescence in situ hybridization (FISH), to determine the rates of gametes with different meiotic contents of translocated chromosomes. Meanwhile, 3030 biopsied blastocysts from 239 male and 293 female reciprocal translocation carriers were detected with the microarray technique to analyze the rates of embryos with different translocated chromosome contents.
The D3 embryo rate, blastocyst formation rate, and euploid rate of blastocysts were remarkably higher in male carriers than those in female (p = 0.001, p = 0.004, and p = 0.035, respectively). In addition, the percentages of alternate products, which contained normal/balanced chromosome contents, in embryos from male carriers were markedly higher than those in sperm FISH (p = 2.48 × 10 and p = 2.88 × 10), while the percentages of adjacent-2 and 3:1 products were lower than those in sperm FISH (p = 0.003 and p = 5.28 × 10). Moreover, consistent results were obtained when comparing the rates of products in embryos between male and female carriers. Specifically, the incidence of alternate products in male carriers was higher than those in female carriers (p = 0.022). However, no similar differences were seen between sperm and embryos of female carriers.
ICSI facilitates the selection of spermatozoa with normal/balanced chromosome contents and improves the D3 embryo rate, blastocyst formation rate, and the euploid embryo rate in male carriers.
评估在显微镜下为胞浆内单精子注射（ICSI）挑选的形态正常的精子，其正常/平衡染色体含量的比率是否高于相互易位携带者未挑选的全部精子。
对40名男性易位携带者每人的500个未挑选精子进行荧光原位杂交（FISH），以确定具有不同减数分裂易位染色体含量的配子比率。同时，用微阵列技术检测来自239名男性和293名女性相互易位携带者的3030个活检囊胚，分析具有不同易位染色体含量的胚胎比率。
男性携带者的D3胚胎率、囊胚形成率和囊胚整倍体率显著高于女性（分别为p = 0.001、p = 0.004和p = 0.035）。此外，男性携带者胚胎中含有正常/平衡染色体含量的交替产物百分比显著高于精子FISH检测结果（p = 2.48×10和p = 2.88×10），而相邻-2和3:1产物的百分比低于精子FISH检测结果（p = 0.003和p = 5.28×10）。此外，比较男性和女性携带者胚胎中的产物比率时得到一致结果。具体而言，男性携带者中交替产物的发生率高于女性携带者（p = 0.022）。然而，女性携带者的精子和胚胎之间未观察到类似差异。
ICSI有助于挑选具有正常/平衡染色体含量的精子，并提高男性携带者的D3胚胎率、囊胚形成率和整倍体胚胎率。
