Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Cardiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Physiol Rep. 2020 Nov;8(22):e14637. doi: 10.14814/phy2.14637.
Frequencies of circulating T follicular helper (cTfh) functional subsets vary in autoimmune diseases. We evaluated the frequencies and clinical relevance of functional subsets of cTfhs in patients with different degrees of stenosis. Blood samples were collected from high (≥50%) (n = 12) and low (<50%) stenosis (n = 12) groups and healthy controls (n = 6). Three subsets of cTfh cells including cTfh1 (CXCR3 CCR6 ), cTfh2 (CXCR3 CCX6 ), and cTfh17 (CXCR3 CCR6 ) were detected by flow cytometry. The frequency of cTfh1 cells was higher in control (p = .0006) and low-stenosis groups (p = .005) compared to high-stenosis group. The percentages of cTfh2 and cTfh17 cells were increased in high-stenosis compared to low-stenosis (p = .002 and p = .007) and control groups (p = .0004 and p = .0005), respectively. The frequency of cTfh1 cells negatively correlated with cholesterol (p = .040; r = -.44), C-reactive protein (CRP) (p = .015; r = -.68), erythrocyte sedimentation rate (ESR) (p = .002; r = -.79), neutrophil/lymphocyte ratio (NLR) (p = .028; r = -.67), and cTfh17 (p = .017; r = -.7244) in the high-stenosis group. The percentages of cTfh2 and cTfh17 cells positively correlated with cholesterol (p = .025; r = .77 and p = .033; r = .71), CRP (p = .030; r = .61 and p = .020; r = .73), ESR (p = .027; r = .69 and p = .029; r = .70), NLR (p = .004; r = .76 and p = .005; r = .74), and with each other (p = .022; r = .7382), respectively, in the high-stenosis group. The increased frequencies of cTfh2 and cTfh17 subsets and their correlation with laboratory parameters in patients with atherosclerosis may suggest their role in promoting the inflammatory response and atherosclerosis progression.
循环滤泡辅助性 T 细胞（cTfh）功能亚群的频率在自身免疫性疾病中有所不同。我们评估了不同狭窄程度患者的 cTfhs 功能亚群的频率及其临床相关性。从高（≥50%）狭窄（n=12）和低（<50%）狭窄（n=12）组以及健康对照组（n=6）采集血样。通过流式细胞术检测 cTfh1（CXCR3 CCR6）、cTfh2（CXCR3 CCX6）和 cTfh17（CXCR3 CCR6）三种 cTfh 细胞亚群。与高狭窄组相比，对照组（p=0.0006）和低狭窄组（p=0.005）的 cTfh1 细胞频率更高。与低狭窄组（p=0.002 和 p=0.007）和对照组（p=0.0004 和 p=0.0005）相比，高狭窄组中 cTfh2 和 cTfh17 细胞的百分比增加。高狭窄组中 cTfh1 细胞的频率与胆固醇（p=0.040；r=-0.44）、C 反应蛋白（CRP）（p=0.015；r=-0.68）、红细胞沉降率（ESR）（p=0.002；r=-0.79）、中性粒细胞/淋巴细胞比值（NLR）（p=0.028；r=-0.67）和 cTfh17（p=0.017；r=-0.7244）呈负相关。高狭窄组中 cTfh2 和 cTfh17 细胞的百分比与胆固醇（p=0.025；r=0.77 和 p=0.033；r=0.71）、CRP（p=0.030；r=0.61 和 p=0.020；r=0.73）、ESR（p=0.027；r=0.69 和 p=0.029；r=0.70）、NLR（p=0.004；r=0.76 和 p=0.005；r=0.74）和彼此之间（p=0.022；r=0.7382）呈正相关。动脉粥样硬化患者 cTfh2 和 cTfh17 亚群频率的增加及其与实验室参数的相关性可能提示它们在促进炎症反应和动脉粥样硬化进展中的作用。
