Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China; First Clinical College, Fujian Medical University, Fuzhou, China; Fujian Key Laboratory of Laboratory Medicine, Fuzhou, China.
Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Clin Chim Acta. 2020 Jan;500:149-154. doi: 10.1016/j.cca.2019.10.009. Epub 2019 Oct 28.
The albumin to fibrinogen ratio (AFR) and the C-reactive protein to albumin ratio (CAR) have been served as inflammatory markers. However, their roles in RA remain unclear. We investigated the association of AFR/CAR with the concentration of autoantibodies and Th17 cells in RA.
A total of 196 RA patients, 200 patients with systemic lupus erythematosus (SLE), and 200 healthy donors (HD) who were admitted to the First Affiliated Hospital of Fujian Medical University were enrolled. The results of FIB, ALB, CRP, anti-cyclic citrullinated peptide antibodies (anti-CCP), rheumatoid factor (RF) and erythrocyte sedimentation rate (ESR) from RA patients and SLE patients were retrospectively analyzed. The percentage of Th17 cells in peripheral blood of RA patients was detected by flow cytometry, and the relative expression of TNF-α, IL-6 and IL-17A was detected by RT-qPCR. Correlation analysis of AFR/CAR and Th17 cells, CRP, ESR, TNF-α, IL-6 and IL-17A in RA was conducted.
Compared with SLE patients and healthy donors (HD), AFR concentration was significantly lower (P < 0.01) in RA patients, while CAR concentration was significantly increased (P < 0.01) in RA patients. AFR showed negative correlation with CRP (r = -0.7103), ESR (r = -0.6542), RF (-0.2219), Th17 cells (r = -0.5952) and IL-17A (r = -0.4681). CAR was positively correlated with CRP (r = 0.9899), ESR (r = 0.605), RF (0.1867), Th17 cells (r = 0.6818), TNF-α (r = 0.3388), and IL-17A (r = 0.2046).
The concentration of AFR in RA patients was reduced, while CAR concentration was increased. AFR and CAR are associated with CRP, ESR, RF, and Th17 cell ratios in RA patients, which can be used as potential indicators for determining RA inflammation.
白蛋白与纤维蛋白原比值（AFR）和 C 反应蛋白与白蛋白比值（CAR）已被用作炎症标志物。然而，它们在类风湿关节炎（RA）中的作用仍不清楚。我们研究了 AFR/CAR 与 RA 患者自身抗体和 Th17 细胞浓度的关系。
共纳入 196 例 RA 患者、200 例系统性红斑狼疮（SLE）患者和 200 名健康对照者（HD），均来自福建医科大学附属第一医院。回顾性分析 RA 患者和 SLE 患者的纤维蛋白原（FIB）、白蛋白（ALB）、C 反应蛋白（CRP）、抗环瓜氨酸肽抗体（抗-CCP）、类风湿因子（RF）和红细胞沉降率（ESR）结果。采用流式细胞术检测 RA 患者外周血 Th17 细胞的百分比，采用 RT-qPCR 检测 TNF-α、IL-6 和 IL-17A 的相对表达量。对 RA 患者的 AFR/CAR 与 Th17 细胞、CRP、ESR、TNF-α、IL-6 和 IL-17A 进行相关性分析。
与 SLE 患者和健康对照者（HD）相比，RA 患者的 AFR 浓度明显降低（P<0.01），而 CAR 浓度明显升高（P<0.01）。AFR 与 CRP（r=-0.7103）、ESR（r=-0.6542）、RF（-0.2219）、Th17 细胞（r=-0.5952）和 IL-17A（r=-0.4681）呈负相关。CAR 与 CRP（r=0.9899）、ESR（r=0.605）、RF（0.1867）、Th17 细胞（r=0.6818）、TNF-α（r=0.3388）和 IL-17A（r=0.2046）呈正相关。
RA 患者的 AFR 浓度降低，而 CAR 浓度升高。AFR 和 CAR 与 RA 患者的 CRP、ESR、RF 和 Th17 细胞比值相关，可作为判断 RA 炎症的潜在指标。
