Integrated Islet Distribution Program, Department of Diabetes & Cancer Discovery Science, City of Hope, Duarte, CA, USA.
Department of Surgery, University of California Irvine, Orange, CA, USA.
Cell Transplant. 2020 Jan-Dec;29:963689720974582. doi: 10.1177/0963689720974582.
Access to functional high-quality pancreatic human islets is critical to advance diabetes research. The Integrated Islet Distribution Program (IIDP), a major source for human islet distribution for over 15 years, conducted a study to evaluate the most advantageous times to ship islets postisolation to maximize islet recovery. For the evaluation, three experienced IIDP Islet Isolation Centers each provided samples from five human islet isolations, shipping 10,000 islet equivalents (IEQ) at four different time periods postislet isolation (no 37°C culture and shipped within 0 to 18 hours; or held in 37°C culture for 18 to 42, 48 to 96, or 144 to 192 hours). A central evaluation center compared samples for islet quantity, quality, and viability for each experimental condition preshipment and postshipment, as well as post 37°C culture 18 to 24 hours after shipment receipt. Additional evaluations included measures of functional potency by static glucose-stimulated insulin release (GSIR), represented as a stimulation index. Comparing the results of the four preshipment holding periods, the greatest IEQ loss postshipment occurred with the shortest preshipment times. Similar patterns emerged when comparing preshipment to postculture losses. islet function (GSIR) was not adversely impacted by increased tissue culture time. These data indicate that allowing time for islet recovery postisolation, prior to shipping, yields less islet loss during shipment without decreasing islet function.
获取功能齐全的高质量人胰腺胰岛是推进糖尿病研究的关键。IIDP（胰岛综合分配计划）作为人胰岛分配的主要来源已经超过 15 年，进行了一项研究来评估最有利于胰岛分离后运输的时间，以最大限度地提高胰岛回收率。在这项评估中，三个经验丰富的 IIDP 胰岛分离中心各提供了五个胰岛分离样本，在四个不同的时间点运输了 10000 个胰岛当量（IEQ），分别是胰岛分离后 0 至 18 小时（无 37°C 培养且在 18 小时内运输），或在 37°C 培养 18 至 42 小时、48 至 96 小时或 144 至 192 小时。一个中央评估中心在运输前和运输后以及运输后 37°C 培养 18 至 24 小时时，比较了每个实验条件下样本的胰岛数量、质量和活力。额外的评估包括通过静态葡萄糖刺激胰岛素释放（GSIR）来衡量功能效力，以刺激指数表示。比较四个运输前保存期的结果，发现运输后 IEQ 损失最大的是运输前时间最短的。在比较运输前和培养后损失时，出现了类似的模式。胰岛功能（GSIR）不受组织培养时间增加的不利影响。这些数据表明，在运输前允许胰岛在分离后恢复一段时间，可在运输过程中减少胰岛损失，而不会降低胰岛功能。
