Department of Animal and Poultry Science, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan S7N 5A8, Canada.
London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, Ontario N5V 4T3, Canada.
J Agric Food Chem. 2020 Dec 9;68(49):14540-14548. doi: 10.1021/acs.jafc.0c02508. Epub 2020 Nov 24.
This study aimed to explore the comparative effects of overexpressing miR156 with individually silencing and genes on carbohydrate physiochemical, fermentation, and nutritional profiles of alfalfa (). Three sub-genotypes of miR156 overexpressed (miR156 OE), SPL6RNAi, and SPL13RNAi grown with the wild type (WT) in a greenhouse were harvested 3 times at an early vegetative stage. Samples were freeze-dried, ground, and analyzed for carbohydrate nutritional profiles in terms of chemical composition, CNCPS fractions, energetic values, degradation, and fermentation characteristics. Results showed that miR156 OE had lower fiber and higher energy compared to all other genotypes. Moreover, miR156 OE had higher starch compared to SPL13RNAi and higher DM degradation compared to WT and SPL13RNAi. In conclusion, overexpression of miR156 decreased the fiber content of alfalfa but increased energy and DM degradation. SPL6RNAi was more similar to miR156 OE alfalfa in chemical composition and degradation, indicating that the gene plays an important role in the miR156 overexpression event.
本研究旨在探讨过表达 miR156 与单独沉默基因和基因对紫花苜蓿（）碳水化合物理化特性、发酵和营养特性的比较影响。三种 miR156 过表达亚基因型（miR156 OE）、SPL6RNAi 和 SPL13RNAi 与野生型（WT）在温室中生长，在早期营养阶段收获 3 次。将样品冻干、粉碎，并分析碳水化合物营养成分的化学成分、CNCPS 分数、能量值、降解和发酵特性。结果表明，与所有其他基因型相比，miR156 OE 的纤维含量较低，能量较高。此外，miR156 OE 的淀粉含量高于 SPL13RNAi，DM 降解率高于 WT 和 SPL13RNAi。总之，miR156 的过表达降低了紫花苜蓿的纤维含量，但增加了能量和 DM 降解。SPL6RNAi 在化学成分和降解方面与 miR156 OE 苜蓿更为相似，表明基因在 miR156 过表达事件中发挥了重要作用。
