miR156/SPL 模块在紫花苜蓿涝害响应中的作用。
Involvement of the miR156/SPL module in flooding response in Medicago sativa.
机构信息
Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON, N5V 4T3, Canada.
Department of Biology, University of Western Ontario, 1151 Richmond Street, London, ON, N6A5B7, Canada.
出版信息
Sci Rep. 2021 Feb 5;11(1):3243. doi: 10.1038/s41598-021-82450-7.
The highly conserved plant microRNA, miR156, affects plant development, metabolite composition, and stress response. Our previous research revealed the role of miR156 in abiotic stress response in Medicago sativa exerted by downregulating SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE transcription factors. Here we investigated the involvement and possible mechanism of action of the miR156/SPL module in flooding tolerance in alfalfa. For that, we used miR156 overexpressing, SPL13RNAi, flood-tolerant (AAC-Trueman) and -sensitive (AC-Caribou) alfalfa cultivars exposed to flooding. We also used Arabidopsis ABA insensitive (abi1-2, abi5-8) mutants and transgenic lines with either overexpressed (KIN10-OX1, KIN10-OX2) or silenced (KIN10RNAi-1, KIN10RNAi-2) catalytic subunit of SnRK1 to investigate a possible role of ABA and SnRK1 in regulating miR156 expression under flooding. Physiological analysis, hormone profiling and global transcriptome changes revealed a role for miR156/SPL module in flooding tolerance. We also identified nine novel alfalfa SPLs (SPL1, SPL1a, SPL2a, SPL7, SPL7a, SPL8, SPL13a, SPL14, SPL16) responsive to flooding. Our results also showed a possible ABA-dependent SnRK1 upregulation to enhance miR156 expression, resulting in downregulation of SPL4, SPL7a, SPL8, SPL9, SPL13, and SPL13a. We conclude that these effects induce flooding adaptive responses in alfalfa and modulate stress physiology by affecting the transcriptome, ABA metabolites and secondary metabolism.
高度保守的植物 microRNA，miR156，影响植物发育、代谢物组成和应激反应。我们之前的研究表明，miR156 通过下调 SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE 转录因子，在紫花苜蓿中发挥非生物胁迫反应的作用。在这里，我们研究了 miR156/SPL 模块在苜蓿耐淹水过程中的参与和可能的作用机制。为此，我们使用了 miR156 过表达、SPL13RNAi、耐淹水（AAC-Trueman）和敏感（AC-Caribou）苜蓿品种暴露在淹水中。我们还使用了拟南芥 ABA 不敏感（abi1-2、abi5-8）突变体和过表达（KIN10-OX1、KIN10-OX2）或沉默（KIN10RNAi-1、KIN10RNAi-2）SnRK1 催化亚基的转基因系来研究 ABA 和 SnRK1 在调节 miR156 表达中的可能作用在洪水下。生理分析、激素分析和全转录组变化表明 miR156/SPL 模块在耐淹水方面的作用。我们还鉴定了 9 个新的苜蓿 SPLs（SPL1、SPL1a、SPL2a、SPL7、SPL7a、SPL8、SPL13a、SPL14、SPL16）对洪水响应。我们的结果还表明，ABA 依赖性 SnRK1 的上调可能增强 miR156 的表达，导致 SPL4、SPL7a、SPL8、SPL9、SPL13 和 SPL13a 的下调。我们得出结论，这些效应诱导苜蓿适应洪水的适应反应，并通过影响转录组、ABA 代谢物和次生代谢来调节应激生理。
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