Donna A, Betta P G, Robutti F
Department of Pathology, City Hospital, Alessandria, Italy.
J Clin Pathol. 1987 Dec;40(12):1428-31. doi: 10.1136/jcp.40.12.1428.
Nine malignant mesotheliomas and 12 specimens of benign reactive mesothelial hyperplasia were examined using a specific antimesothelial cell antibody. Immunostaining intensity was subsequently estimated by means of image analysis. The mean and standard deviation of integrated optical density in the malignant mesotheliomas differed significantly from those in the mesothelial hyperplasias. In all mesotheliomas but one the integrated optical density was greater than in reactive mesothelial hyperplasia. No significant difference in optical density was observed between the two groups; the standard deviation was significantly higher in the reactive lesions. This technique may be adopted to complement the traditional morphological assessment of primary lesions of the serosal cavities.
使用一种特异性抗间皮细胞抗体对9例恶性间皮瘤和12例良性反应性间皮增生标本进行了检测。随后通过图像分析估计免疫染色强度。恶性间皮瘤的积分光密度均值和标准差与间皮增生的均值和标准差有显著差异。除1例外，所有间皮瘤的积分光密度均高于反应性间皮增生。两组间光密度无显著差异；反应性病变的标准差显著更高。该技术可用于补充浆膜腔原发性病变的传统形态学评估。
