Donna A, Betta P G, Bellingeri D, Marchesini A
J Clin Pathol. 1986 Sep;39(9):961-8. doi: 10.1136/jcp.39.9.961.
An antibody was raised against a protein isolated from the cytoplasm of mesothelioma cells. It was subsequently used in an immunoperoxidase procedure on formalin fixed, paraffin embedded tissue sections. A representative sample of benign and malignant tumours from all the systems of the human body was examined. All the tumours derived from coelomic surfaces (mesotheliomas of pleura, peritoneum, and ovary, and adenomatoid tumour of epididymis) reacted with the antibody. No other tumour tested in this study expressed the protein. These findings indicate that the antibody may be useful in the identification of mesothelioma cells in both histological and cytological diagnostic routine practice when morphological interpretation is in doubt.
制备了一种针对从间皮瘤细胞胞质中分离出的一种蛋白质的抗体。随后将其用于对福尔马林固定、石蜡包埋的组织切片进行免疫过氧化物酶检测。对来自人体所有系统的良性和恶性肿瘤的代表性样本进行了检查。所有源自体腔表面的肿瘤（胸膜、腹膜和卵巢的间皮瘤以及附睾的腺瘤样肿瘤）均与该抗体发生反应。本研究中检测的其他肿瘤均未表达该蛋白质。这些发现表明，当形态学解释存在疑问时，该抗体在组织学和细胞学诊断常规实践中可能有助于识别间皮瘤细胞。
