Center of Life Sciences, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia.
Institute of Translational Medicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia.
Int J Mol Sci. 2020 Nov 20;21(22):8800. doi: 10.3390/ijms21228800.
Genetically encoded photosensitizers are increasingly used as optogenetic tools to control cell fate or trigger intracellular processes. A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity-based applications. Here, we applied directed evolution to this protein and identified SuperNova2, a protein with S10R substitution that results in enhanced brightness, chromophore maturation and phototoxicity in bacterial and mammalian cell cultures.
基因编码的光敏剂越来越多地被用作光遗传学工具来控制细胞命运或触发细胞内过程。最近开发了一种称为 SuperNova 的单体红色荧光蛋白，然而，它在大多数基于光毒性的应用中表现出不理想的特性。在这里，我们对该蛋白进行了定向进化，鉴定出 SuperNova2，这是一种 S10R 取代的蛋白，可提高细菌和哺乳动物细胞培养物中的亮度、生色团成熟度和光毒性。
