Université Paris-Saclay, CNRS, Institut de Chimie des Substances Naturelles, UPR 2301, 91198, Gif-sur-Yvette, France.
Université Paris-Saclay, CNRS, Institut de Chimie des Substances Naturelles, UPR 2301, 91198, Gif-sur-Yvette, France.
J Chromatogr A. 2020 Dec 20;1634:461703. doi: 10.1016/j.chroma.2020.461703. Epub 2020 Nov 13.
The internal energy distributions of thermometer ions dissolved in supercritical and liquid solvents and produced in the gas phase by electrospray (ESI) were measured and compared by the survival yield method. The influence of different chromatographic conditions such as the nature of solvents, the composition of the mobile phase, the pressure of the back pressure regulator was studied for supercritical fluid chromatography (SFC) whereas the influence of the composition and of the flow rate of the mobile phase was investigated for liquid chromatography (LC). The MS instrumental parameters were studied in parallel for SFC and LC showing that the drying gas temperature and the fragmentor voltage affected the internal energy distribution, whereas the capillary voltage did not modify the internal energy distribution. A comparison of the internal energy distributions generated by SFC and LC was carried out leading to conclude that SFC led to higher internal energy, i.e. more in-source fragment ions, than LC.
采用存活产率法测量并比较了溶解在超临界和液相溶剂中、通过电喷雾（ESI）在气相中产生的温度计离子的内部分布。研究了不同的色谱条件对超临界流体色谱（SFC）的影响，如溶剂的性质、流动相的组成、背压调节阀的压力；对液相色谱（LC）的影响，如流动相的组成和流速。同时对 SFC 和 LC 的 MS 仪器参数进行了研究，结果表明干燥气体温度和碎裂电压影响内部分布，而毛细管电压不改变内部分布。对 SFC 和 LC 产生的内部分布进行了比较，得出结论：SFC 比 LC 产生更高的内能，即更多的源内碎裂离子。
