• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

转录组分析揭示了大花猪毛菜种子发育过程中油脂积累的动态特性。

Transcriptome analyses reveals the dynamic nature of oil accumulation during seed development of Plukenetia volubilis L.

机构信息

China Eucalypt Research Centre (CERC), Zhanjiang, 524022, Guangdong, China.

出版信息

Sci Rep. 2020 Nov 24;10(1):20467. doi: 10.1038/s41598-020-77177-w.

DOI:10.1038/s41598-020-77177-w
PMID:33235240
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7686490/
Abstract

Sacha inchi (Plukenetia volubilis L.) is a shrub native to Amazon rainforests that's of commercial interest as its seeds contain 35-60% edible oil (dry weight). This oil is one of the healthiest vegetable oils due to its high polyunsaturated fatty acid content and favourable ratio of omega-6 to omega-3 fatty acids. De novo transcriptome assembly and comparative analyses were performed on sacha inchi seeds from five stages of seed development in order to identifying genes associated with oil accumulation and fatty acid production. Of 30,189 unigenes that could be annotated in public databases, 20,446 were differentially expressed unigenes. A total of 14 KEGG pathways related to lipid metabolism were found, and 86 unigenes encoding enzymes involved in α-linolenic acid (ALA) biosynthesis were obtained including five unigenes encoding FATA (Unigene0008403), SAD (Unigene0012943), DHLAT (Unigene0014324), α-CT (Unigene0022151) and KAS II (Unigene0024371) that were significantly up-regulated in the final stage of seed development. A total of 66 unigenes encoding key enzymes involved in the synthesis of triacylglycerols (TAGs) were found, along with seven unigenes encoding PDCT (Unigene0000909), LPCAT (Unigene0007846), Oleosin3 (Unigene0010027), PDAT1 (Unigene0016056), GPDH (Unigene0022660), FAD2 (Unigene0037808) and FAD3 (Unigene0044238); these also proved to be up-regulated in the final stage of seed development.

摘要

沙棘（Plukenetia volubilis L.）是一种原产于亚马逊雨林的灌木，因其种子含有 35-60%可食用油（干重）而具有商业价值。这种油是最健康的植物油之一，因为它含有高比例的多不饱和脂肪酸和 ω-6 与 ω-3 脂肪酸的理想比例。为了鉴定与油脂积累和脂肪酸生成相关的基因，我们对来自五个种子发育阶段的沙棘种子进行了从头转录组组装和比较分析。在可以注释到公共数据库的 30189 个 unigenes 中，有 20446 个是差异表达的 unigenes。共发现与脂质代谢相关的 14 条 KEGG 途径，获得了 86 个编码参与 α-亚麻酸（ALA）生物合成的酶的 unigenes，包括 5 个编码 FATA（Unigene0008403）、SAD（Unigene0012943）、DHLAT（Unigene0014324）、α-CT（Unigene0022151）和 KAS II（Unigene0024371）的 unigenes，它们在种子发育的最后阶段显著上调。还发现了 66 个编码参与三酰基甘油（TAG）合成的关键酶的 unigenes，以及 7 个编码 PDCT（Unigene0000909）、LPCAT（Unigene0007846）、油体蛋白 3（Unigene0010027）、PDAT1（Unigene0016056）、GPDH（Unigene0022660）、FAD2（Unigene0037808）和 FAD3（Unigene0044238）的 unigenes；这些基因在种子发育的最后阶段也被证明上调。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/f669a7681525/41598_2020_77177_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/ae5043992542/41598_2020_77177_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/8e9998dd9598/41598_2020_77177_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/8a43c66ec6bc/41598_2020_77177_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/6c4d92a41845/41598_2020_77177_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/364f86209d7f/41598_2020_77177_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/e832f7f9ed3e/41598_2020_77177_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/cc3a1167ba81/41598_2020_77177_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/541558fcbc6a/41598_2020_77177_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/e3c508365f21/41598_2020_77177_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/f669a7681525/41598_2020_77177_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/ae5043992542/41598_2020_77177_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/8e9998dd9598/41598_2020_77177_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/8a43c66ec6bc/41598_2020_77177_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/6c4d92a41845/41598_2020_77177_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/364f86209d7f/41598_2020_77177_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/e832f7f9ed3e/41598_2020_77177_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/cc3a1167ba81/41598_2020_77177_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/541558fcbc6a/41598_2020_77177_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/e3c508365f21/41598_2020_77177_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46c5/7686490/f669a7681525/41598_2020_77177_Fig10_HTML.jpg

相似文献

1
Transcriptome analyses reveals the dynamic nature of oil accumulation during seed development of Plukenetia volubilis L.转录组分析揭示了大花猪毛菜种子发育过程中油脂积累的动态特性。
Sci Rep. 2020 Nov 24;10(1):20467. doi: 10.1038/s41598-020-77177-w.
2
De novo transcriptome assembly of the eight major organs of Sacha Inchi (Plukenetia volubilis) and the identification of genes involved in α-linolenic acid metabolism.藜麦（Plukenetia volubilis）八大器官从头转录组组装及参与α-亚麻酸代谢基因的鉴定。
BMC Genomics. 2018 May 22;19(1):380. doi: 10.1186/s12864-018-4774-y.
3
Expression of genes controlling unsaturated fatty acids biosynthesis and oil deposition in developing seeds of Sacha inchi (Plukenetia volubilis L.).控制美藤果（南美油藤）发育种子中不饱和脂肪酸生物合成和油脂沉积的基因表达
Lipids. 2014 Oct;49(10):1019-31. doi: 10.1007/s11745-014-3938-z. Epub 2014 Aug 14.
4
Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages.转录组分析 Sacha Inchi（Plukenetia volubilis L.）种子在两个发育阶段。
BMC Genomics. 2012 Dec 20;13:716. doi: 10.1186/1471-2164-13-716.
5
De novo transcriptome assembly and comparative analysis between male and benzyladenine-induced female inflorescence buds of Plukenetia volubilis.雌雄异株大麻雌雄花序转录组从头组装与比较分析。
J Plant Physiol. 2018 Feb;221:107-118. doi: 10.1016/j.jplph.2017.12.006. Epub 2017 Dec 9.
6
Transcriptomic analysis of α-linolenic acid content and biosynthesis in Paeonia ostii fruits and seeds.转录组分析白芍果实和种子中 α-亚麻酸含量与生物合成。
BMC Genomics. 2021 Apr 23;22(1):297. doi: 10.1186/s12864-021-07594-2.
7
Transcriptomic analysis of Perilla frutescens seed to insight into the biosynthesis and metabolic of unsaturated fatty acids.转录组分析紫苏籽以深入了解不饱和脂肪酸的生物合成和代谢。
BMC Genomics. 2018 Mar 21;19(1):213. doi: 10.1186/s12864-018-4595-z.
8
Sacha inchi (Plukenetia volubilis L.): Nutritional composition, biological activity, and uses.萨奇果（Plukenetia volubilis L.）：营养成分、生物活性及用途。
Food Chem. 2018 Nov 1;265:316-328. doi: 10.1016/j.foodchem.2018.05.055. Epub 2018 May 29.
9
Identification of genes associated with the biosynthesis of unsaturated fatty acid and oil accumulation in herbaceous peony 'Hangshao' (Paeonia lactiflora 'Hangshao') seeds based on transcriptome analysis.基于转录组分析鉴定与草本牡丹‘杭芍’（Paeonia lactiflora 'Hangshao'）种子不饱和脂肪酸生物合成和油脂积累相关的基因。
BMC Genomics. 2021 Feb 1;22(1):94. doi: 10.1186/s12864-020-07339-7.
10
Selection of Reliable Reference Genes for Gene Expression Studies of a Promising Oilseed Crop, Plukenetia volubilis, by Real-Time Quantitative PCR.通过实时定量PCR为一种有前景的油料作物南美油藤的基因表达研究选择可靠的内参基因。
Int J Mol Sci. 2015 Jun 3;16(6):12513-30. doi: 10.3390/ijms160612513.

引用本文的文献

1
Origin, evolution and diversification of plant caleosins.植物钙结合蛋白的起源、进化与多样化
BMC Plant Biol. 2025 Apr 5;25(1):433. doi: 10.1186/s12870-025-06463-4.
2
Transcriptional and metabolic analysis of oleic acid synthesis in seedless and tenera oil palm species.无核和杜拉油棕品种油酸合成的转录和代谢分析
Front Plant Sci. 2025 Feb 25;16:1557544. doi: 10.3389/fpls.2025.1557544. eCollection 2025.
3
Sacha Inchi ( L.) Protein Hydrolysate as a New Ingredient of Functional Foods.印加果（L.）蛋白水解物作为功能性食品的新成分

本文引用的文献

1
Characterization and validation of olive FAD and SAD gene families: expression analysis in different tissues and during fruit development.橄榄 FAD 和 SAD 基因家族的特征和验证：不同组织和果实发育过程中的表达分析。
Mol Biol Rep. 2020 Jun;47(6):4345-4355. doi: 10.1007/s11033-020-05554-9. Epub 2020 May 28.
2
Tung Tree (Vernicia fordii) Genome Provides A Resource for Understanding Genome Evolution and Improved Oil Production.油桐（Vernicia fordii）基因组为理解基因组进化和提高产油提供资源。
Genomics Proteomics Bioinformatics. 2019 Dec;17(6):558-575. doi: 10.1016/j.gpb.2019.03.006. Epub 2020 Mar 26.
3
Foods. 2024 Jun 27;13(13):2045. doi: 10.3390/foods13132045.
4
Full-length transcriptome revealed the accumulation of polyunsaturated fatty acids in developing seeds of .全长转录组揭示了在 发育种子中多不饱和脂肪酸的积累。
PeerJ. 2022 Sep 20;10:e13998. doi: 10.7717/peerj.13998. eCollection 2022.
5
Integrated Transcriptome and Proteome Analysis Provides Insight into the Ribosome Inactivating Proteins in Seeds.整合转录组和蛋白质组分析为种子中的核糖体失活蛋白提供了深入了解。
Int J Mol Sci. 2022 Aug 24;23(17):9562. doi: 10.3390/ijms23179562.
6
Transcriptional Basis for Haustorium Formation and Host Establishment in Hemiparasitic Mistletoes.半寄生槲寄生吸器形成和宿主定植的转录基础
Front Genet. 2022 Jun 13;13:929490. doi: 10.3389/fgene.2022.929490. eCollection 2022.
7
Overexpression of Gene from Promotes the Biosynthesis of α-Linolenic Acid in Transgenic Tobacco Seeds.来自[具体来源未明确]的基因过表达促进转基因烟草种子中α-亚麻酸的生物合成。
Genes (Basel). 2022 Feb 28;13(3):450. doi: 10.3390/genes13030450.
Toward understanding the origin and evolution of cellular organisms.
为了理解细胞生物的起源和进化。
Protein Sci. 2019 Nov;28(11):1947-1951. doi: 10.1002/pro.3715. Epub 2019 Sep 9.
4
Expression of a Lychee with an Enhances Cyclopropane Fatty Acid Accumulation in Camelina Seeds.表达荔枝增强了亚麻荠中环丙烷脂肪酸的积累。
Plant Physiol. 2019 Jul;180(3):1351-1361. doi: 10.1104/pp.19.00396. Epub 2019 May 13.
5
Comparative Transcriptome Analysis Reveals an Efficient Mechanism of α-Linolenic Acid in Tree Peony Seeds.比较转录组分析揭示了牡丹籽油中 α-亚麻酸的高效机制。
Int J Mol Sci. 2018 Dec 24;20(1):65. doi: 10.3390/ijms20010065.
6
Fatty acid desaturase 3 (PsFAD3) from Paeonia suffruticosa reveals high α-linolenic acid accumulation.牡丹脂肪酸去饱和酶 3（PsFAD3）具有高α-亚麻酸积累能力。
Plant Sci. 2018 Sep;274:212-222. doi: 10.1016/j.plantsci.2018.05.027. Epub 2018 May 29.
7
De novo transcriptome assembly of the eight major organs of Sacha Inchi (Plukenetia volubilis) and the identification of genes involved in α-linolenic acid metabolism.藜麦（Plukenetia volubilis）八大器官从头转录组组装及参与α-亚麻酸代谢基因的鉴定。
BMC Genomics. 2018 May 22;19(1):380. doi: 10.1186/s12864-018-4774-y.
8
WEGO 2.0: a web tool for analyzing and plotting GO annotations, 2018 update.WEGO 2.0：一个用于分析和绘制 GO 注释的网络工具，2018 年更新。
Nucleic Acids Res. 2018 Jul 2;46(W1):W71-W75. doi: 10.1093/nar/gky400.
9
Carbohydrate reserves and seed development: an overview.碳水化合物储备与种子发育：概述。
Plant Reprod. 2018 Sep;31(3):263-290. doi: 10.1007/s00497-018-0336-3. Epub 2018 May 4.
10
Solvent Extraction and GC-MS Analysis of Sesame Seeds for Determination of Bioactive Antioxidant Fatty Acid/Fatty Oil Components.芝麻的溶剂萃取及气相色谱-质谱联用分析以测定生物活性抗氧化脂肪酸/脂肪油成分
Drug Res (Stuttg). 2018 Jun;68(6):344-348. doi: 10.1055/s-0043-123466. Epub 2017 Dec 12.