Lemus-Conejo Ana, Villanueva-Lazo Alvaro, Martin Maria E, Millan Francisco, Millan-Linares Maria C
Foundation Centre for Research and Development of Functional Food-CIDAF, Avda del Conocimiento 37, 18100 Granade, Spain.
Food Protein and Immunonutrition Group, Department of Food and Health, Instituto de la Grasa, CSIC, Campus Universitario Pablo de Olavide, Edificio 46, Ctra. de Utrera, Km. 1, 41013 Seville, Spain.
Foods. 2024 Jun 27;13(13):2045. doi: 10.3390/foods13132045.
Sacha inchi ( L.) is an under-exploited crop with great potential due to its nutritional and medicinal characteristics. A Sacha inchi protein isolate (SII), obtained from defatted Sacha inchi flour (SIF), was hydrolyzed by Bioprotease LA 660 under specific conditions. The hydrolysates were characterized chemically, and their digestibility and antioxidant capacity were evaluated by in vitro cell-free experiments to select the hydrolysate with major antioxidant activity. Sacha inchi protein hydrolysate at 20 min (SIH20B) was selected, and the anti-inflammatory capacity was evaluated by RT-qPCR and ELISA techniques, using two different doses in monocytes THP-1 stimulated with lipopolysaccharide (LPS). The results obtained showed that the in vitro administration of SIH20B down-regulated the TNF-α gene and reduced the release of this cytokine, whereas the anti-inflammatory cytokines IL-10 and IL-4 were up-regulated in LPS-stimulated monocytes and co-administrated with SIH20B. The peptides contained in SIH20B were identified, and the 20 more relatively abundant peptides with a mass by 1 kDa were subjected to in silico analysis to hypothesize those that could be responsible for the bioactivity reported in the hydrolysate. From the identified peptides, the peptides AAGALKKFL and LGVKFKGGL, among others, are proposed as the most biologically actives. In conclusion, SIH20B is a novel, natural source of high-value-added biopeptides that could be used as an ingredient in formulations of food or nutraceutical compounds.
美藤果（Plukenetia volubilis L.）是一种尚未得到充分开发的作物，因其营养和药用特性而具有巨大潜力。从脱脂美藤果粉（SIF）中获得的美藤果蛋白分离物（SII）在特定条件下被生物蛋白酶LA 660水解。对水解产物进行化学表征，并通过体外无细胞实验评估其消化率和抗氧化能力，以选择具有主要抗氧化活性的水解产物。选择了20分钟时的美藤果蛋白水解产物（SIH20B），并使用两种不同剂量，通过RT-qPCR和ELISA技术在脂多糖（LPS）刺激的单核细胞THP-1中评估其抗炎能力。所得结果表明，体外给予SIH20B可下调TNF-α基因并减少该细胞因子的释放，而抗炎细胞因子IL-10和IL-4在LPS刺激的单核细胞中并与SIH20B共同给药时上调。鉴定了SIH20B中含有的肽，并对质量相差1 kDa的20种相对丰富的肽进行了计算机分析，以推测那些可能对水解产物中报道的生物活性负责的肽。从鉴定出的肽中，提出AAGALKKFL和LGVKFKGGL等肽为最具生物活性的肽。总之，SIH20B是一种新型的、高附加值生物肽的天然来源，可作为食品或营养化合物配方中的一种成分。
