Brasch M A, Meyer R J
Department of Microbiology, University of Texas, Austin 78712.
J Mol Biol. 1987 Dec 5;198(3):361-9. doi: 10.1016/0022-2836(87)90286-5.
oriT, the region required in cis for conjugative mobilization of broad host-range plasmid R1162, has been localized to a 38 base-pair segment of DNA. The oriT DNA is also required for conjugation-dependent recombination. Point mutations at the HinPI cleavage site within oriT affect both mobilization and recombination, and the crossover location has been mapped to this site. An inverted repeat ten base-pairs from the recombination site is also involved in mobilization and recombination, and may be a recognition site for proteins involved in cleavage of the oriT DNA. The properties of conjugation-dependent recombination suggest that mobilization entails the formation of a linear intermediate that is transferred with both a unique origin and polarity.
oriT是广泛宿主范围质粒R1162进行接合转移时顺式作用所需的区域，已被定位到一段38个碱基对的DNA片段上。oriT DNA也是依赖于接合作用的重组所必需的。oriT内HinPI切割位点处的点突变会影响转移和重组，并且交叉位置已被定位到该位点。距重组位点10个碱基对的一个反向重复序列也参与转移和重组，可能是参与oriT DNA切割的蛋白质的识别位点。依赖于接合作用的重组特性表明，转移需要形成一个线性中间体，该中间体以独特的起点和极性进行转移。
