Djordjevic Miodrag, Karanikolic Aleksandar, Velickovic Ljubinka, Milentijevic Maja
Miodrag Djordjevic, Clinic of Endocrine Surgery, Nis, Serbia. Medical Faculty, University Nis, Serbia.
Aleksandar Karanikolic, Clinic of Endocrine Surgery, Nis, Serbia. Medical Faculty, University Nis, Serbia.
Pak J Med Sci. 2020 Nov-Dec;36(7):1539-1544. doi: 10.12669/pjms.36.7.2954.
In order to enhance the prognostic benefit of new molecular markers, the aim of this study was to identify possible association of axillary lymph node (ALN) status and pN with clinicopathological characteristics and expression of EZH2 and CD44 in invasive ductal carcinoma (IDC) of the breast.
The investigation included 106 patients with IDC who had undergone radical mastectomy at the Clinic of Endocrine Surgery in Nis. Clinicopathologic parameters and immunohistochemical expression of EZH2 and CD44 in primary IDC were investigated in relation to ALN status and pN.
Our univariate analysis established that T3-T4 stage, high EZH2, and high EZH2 with ER- were associated with ALN metastasis (p=0.014; 0.003; 0.013). Decreased probability for ALN involvement was found with T1 stage, and low EZH2 with ER+ (p=0.032; 0.022). Multivariant analysis established that high EZH2 in cancer cells was associated with high risk for ALN metastases (p=0.004); T1 tumors were associated with low risk (p=0.037). Higher pN was associated with high EZH2, high EZH2 with ER-, as well as an advanced clinical and disease stage (p=0.006; 0.001; p=0.002, 0.001). Lower pN was associated with ER+, and ER+ with low EZH2 (p= 0.004; 0.012). CD44 was not associated with ALN involvement, nor with pN.
This study revealed association of EZH2 with ALN metastases, where disease stage and expression profiles of EZH2 and ER may have affected regional pN.
为提高新分子标志物的预后价值，本研究旨在确定腋窝淋巴结（ALN）状态和pN与乳腺浸润性导管癌（IDC）的临床病理特征以及EZH2和CD44表达之间的可能关联。
该研究纳入了106例在尼什内分泌外科诊所接受根治性乳房切除术的IDC患者。研究了原发性IDC中EZH2和CD44的临床病理参数及免疫组化表达与ALN状态和pN的关系。
单因素分析表明，T3 - T4期、EZH2高表达以及EZH2高表达且雌激素受体（ER）阴性与ALN转移相关（p = 0.014；0.003；0.013）。发现T1期以及EZH2低表达且ER阳性时ALN受累的可能性降低（p = 0.032；0.022）。多因素分析表明，癌细胞中EZH2高表达与ALN转移高风险相关（p = 0.004）；T1期肿瘤与低风险相关（p = 0.037）。较高的pN与EZH2高表达、EZH2高表达且ER阴性以及临床和疾病晚期相关（p = 0.006；0.001；p = 0.002，0.001）。较低的pN与ER阳性以及ER阳性且EZH2低表达相关（p = 0.004；0.012）。CD44与ALN受累及pN均无关。
本研究揭示了EZH2与ALN转移之间的关联，其中疾病分期以及EZH2和ER的表达谱可能影响区域pN。
