Division of Medical Microbiology and Virology, Providence Health Care, St. Paul's Hospital, 1081 Burrard St., Vancouver, BC, V6Z 1Y6, Canada.
Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.
Eur J Clin Microbiol Infect Dis. 2021 Feb;40(2):447-450. doi: 10.1007/s10096-020-04090-5. Epub 2020 Nov 25.
Due to global shortages of flocked nasopharyngeal swabs and appropriate viral transport media during the COVID-19 pandemic, alternate diagnostic specimens for SARS-CoV-2 detection are sought. The accuracy and feasibility of saliva samples collected and transported without specialized collection devices or media were evaluated. Saliva demonstrated good concordance with paired nasopharyngeal swabs for SARS-CoV-2 detection in 67/74 cases (90.5%), though barriers to saliva collection were observed in long-term care residents and outbreak settings. SARS-CoV-2 RNA was stable in human saliva at room temperature for up to 48 h after initial specimen collection, informing appropriate transport time and conditions.
由于 COVID-19 大流行期间，全球鼻咽拭子和适当的病毒运输介质短缺，因此正在寻找用于 SARS-CoV-2 检测的替代诊断标本。评估了未使用专门收集设备或介质收集和运输的唾液样本的准确性和可行性。唾液样本与 74 个配对鼻咽拭子中的 67 个（90.5%）在 SARS-CoV-2 检测方面具有良好的一致性，尽管在长期护理居民和暴发环境中观察到唾液收集存在障碍。SARS-CoV-2 RNA 在室温下从初始标本采集后最长 48 小时内在人唾液中保持稳定，为适当的运输时间和条件提供了信息。
