Department of Infection Prevention and Infectious Diseases, University Medical Center Regensburg, 93053 Regensburg, Germany.
Institute of Medical Microbiology and Hygiene, University of Regensburg, 93053 Regensburg, Germany.
Viruses. 2021 Apr 10;13(4):653. doi: 10.3390/v13040653.
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA is detected by reverse-transcription quantitative real-time PCR (RT-qPCR) from respiratory specimens. This study compares throat washings (TW), nasopharyngeal swabs (NS) and oropharyngeal swabs (OS). A total of 102 samples from 34 adult patients with confirmed SARS-CoV-2 infection were analysed by RT-qPCR with absolute quantification. The median concentrations and diagnostic sensitivities were 5.8×104 copies/mL, 85% (NS), 1.4×104, 79% (OS) and 4.3×103, 85% (TW). Concentration differences were significant between NS and TW ( = 0.019). Saliva (SA) was available from 21 patients (median 3.4×103). OS and TW can be considered for SARS-CoV-2 diagnostics, although with slightly lower concentrations.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的 RNA 可通过呼吸道标本的逆转录实时定量 PCR（RT-qPCR）检测。本研究比较了咽拭子（TW）、鼻咽拭子（NS）和口咽拭子（OS）。对 34 例确诊 SARS-CoV-2 感染的成年患者的 102 份样本进行了 RT-qPCR 绝对定量分析。中位数浓度和诊断灵敏度分别为 5.8×104 拷贝/mL、85%（NS）、1.4×104、79%（OS）和 4.3×103、85%（TW）。NS 和 TW 之间的浓度差异有统计学意义（ = 0.019）。21 例患者可获得唾液（SA）（中位数 3.4×103）。OS 和 TW 可用于 SARS-CoV-2 诊断，尽管浓度略低。
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