咽拭子、鼻咽拭子和口咽拭子检测 SARS-CoV-2 的比较。
Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2.
机构信息
Department of Infection Prevention and Infectious Diseases, University Medical Center Regensburg, 93053 Regensburg, Germany.
Institute of Medical Microbiology and Hygiene, University of Regensburg, 93053 Regensburg, Germany.
出版信息
Viruses. 2021 Apr 10;13(4):653. doi: 10.3390/v13040653.
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA is detected by reverse-transcription quantitative real-time PCR (RT-qPCR) from respiratory specimens. This study compares throat washings (TW), nasopharyngeal swabs (NS) and oropharyngeal swabs (OS). A total of 102 samples from 34 adult patients with confirmed SARS-CoV-2 infection were analysed by RT-qPCR with absolute quantification. The median concentrations and diagnostic sensitivities were 5.8×104 copies/mL, 85% (NS), 1.4×104, 79% (OS) and 4.3×103, 85% (TW). Concentration differences were significant between NS and TW ( = 0.019). Saliva (SA) was available from 21 patients (median 3.4×103). OS and TW can be considered for SARS-CoV-2 diagnostics, although with slightly lower concentrations.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的 RNA 可通过呼吸道标本的逆转录实时定量 PCR（RT-qPCR）检测。本研究比较了咽拭子（TW）、鼻咽拭子（NS）和口咽拭子（OS）。对 34 例确诊 SARS-CoV-2 感染的成年患者的 102 份样本进行了 RT-qPCR 绝对定量分析。中位数浓度和诊断灵敏度分别为 5.8×104 拷贝/mL、85%（NS）、1.4×104、79%（OS）和 4.3×103、85%（TW）。NS 和 TW 之间的浓度差异有统计学意义（ = 0.019）。21 例患者可获得唾液（SA）（中位数 3.4×103）。OS 和 TW 可用于 SARS-CoV-2 诊断，尽管浓度略低。
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