Wang Zheng, Zhou Xue-Dong, Ren Biao
State Key Laboratory of Oral Diseases,National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2020 Nov;51(6):742-748. doi: 10.12182/20201160203.
To investigate the effect of ( ) on proliferation and virulence of ( ), and to verify the role of the ergosterol pathway in it.
After single- and co-cultivation of wild-type 5314 and UA159, the absorbance value of OD and colony-forming units (CFU) were detected to reflect the influence of on the growth of . To reflect the influence of on the virulence of , the production of extracellular polysaccharides was detected by anthrone-vitriol method, and the acid production capacity was detected by a pH meter. After single- and co-cultivation of mutant strains and , the growth of was evaluated by CFU. After inhibiting the ergosterol pathway by 0 mg/L, 0.012 5 mg/L, and 0.025 mg/L fluconazole for 24 h, the CFU of single- and co-cultivated wild-type and were detected.
The OD absorbance value and CFU in theco-cultivation of . wild-type and was higher than that in the single culture ( <0.05), and the production of extracellular polysaccharides in was increased when was co-cultured with ( <0.05), accompanied with a more obvious decrease of pH ( <0.05). Fourteen strains in whole 42 mutant strains lost the growth-promoting effect on , including 6 ergosterol synthesis-related mutant strains. After co-cultivation of the 6 ergosterol synthesis-related mutant strains and , the CFU of remained unchanged or decreased. After inhibiting the ergosterol pathway by 0.012 5 mg/L and 0.025 mg/L fluconazole, the CFU of in the co-cultivation of wild-type and was lower than that without fluconazole treatment ( <0.05), while the CFU of and single-cultivations did not change significantly ( >0.05) and the CFU of in the co-cultivation of wild-type and did not change significantly ( >0.05).
can enhance the growth ability and virulence of through the ergosterol-related pathway. This process can be inhibited by fluconazole, which is expected to become a novel strategy to prevent and treat dental caries.
研究（ ）对（ ）增殖和毒力的影响，并验证麦角固醇途径在其中的作用。
将野生型5314和UA159进行单培养和共培养后，检测OD吸光度值和菌落形成单位（CFU），以反映（ ）对（ ）生长的影响。为反映（ ）对（ ）毒力的影响，采用蒽酮 - 硫酸法检测胞外多糖的产生，并使用pH计检测产酸能力。将突变菌株和（ ）进行单培养和共培养后，通过CFU评估（ ）的生长情况。用0 mg/L、0.012 5 mg/L和0.025 mg/L氟康唑抑制麦角固醇途径24小时后，检测野生型（ ）和（ ）单培养及共培养的CFU。
野生型（ ）和（ ）共培养时的OD吸光度值和CFU高于单培养（P<0.05），（ ）与（ ）共培养时（ ）胞外多糖的产生增加（P<0.05），同时pH下降更明显（P<0.05）。42株突变菌株中有14株对（ ）失去促生长作用，其中包括6株与麦角固醇合成相关的突变菌株。6株与麦角固醇合成相关的突变菌株与（ ）共培养后，（ ）的CFU保持不变或下降。用0.012 5 mg/L和0.025 mg/L氟康唑抑制麦角固醇途径后，野生型（ ）和（ ）共培养时（ ）的CFU低于未用氟康唑处理时（P<0.05），而（ ）单培养和（ ）单培养的CFU无明显变化（P>0.05），野生型（ ）和（ ）共培养时（ ）的CFU也无明显变化（P>0.05）。
（ ）可通过麦角固醇相关途径增强（ ）的生长能力和毒力。这一过程可被氟康唑抑制，有望成为预防和治疗龋齿的新策略。
