Philip Nebu, Leishman Shaneen J, Bandara Hmhn, Walsh Laurence J
Dr. Philip is a doctoral research candidate, the School of Dentistry, University of Queensland, Brisbane, Australia;, Email:
Dr. Leishman is a postdoctoral fellow, the School of Dentistry, University of Queensland, Brisbane, Australia.
Pediatr Dent. 2019 Jan 15;41(1):56-62.
The purpose of this study was to investigate the effects of polyphenol-rich cranberry extracts on dual-species biofilms implicated in contributing to the severity of early childhood caries. biofilms were grown on saliva-coated hydroxyapatite discs (s-HA) mounted on the high-throughput Amsterdam Active Attachment model. The s-HA discs were treated with the cranberry extracts/vehicle control for five minutes just before biofilm growth and subsequently, for similar exposure times, after 12 hours and 24 hours of biofilm growth. The treated 24-hour-old biofilms were then assessed for acidogenicity, metabolic activity, exopolysaccharide (EPS)/microbial biovolumes, structural organization, and colony forming unit (CFU) counts. Treatment with 500 to 1,000 μg/mL of the cranberry extracts produced significant reductions in acidogenicity and metabolic activity (P<0.0001) compared to the control-treated biofilms. A significant decrease in biovolumes of the EPS (P=0.003) and microbial biofilm components (P=0.007) was also seen. Qualitative assessment of confocal biofilm images revealed that the cranberry extract disrupted biofilm structural architecture. Finally, significantly fewer S. mutans (P=0.006) and C. albicans (P=0.036) CFUs were recovered from the cranberry-treated biofilms than from the control-treated bio-films. Conclusions: Cranberry extracts inhibited cariogenic virulence properties of dual-species biofilms in an in vitro model.
本研究的目的是调查富含多酚的蔓越莓提取物对与幼儿龋齿严重程度相关的双物种生物膜的影响。生物膜在安装于高通量阿姆斯特丹主动附着模型上的唾液包被羟基磷灰石圆盘（s-HA）上生长。在生物膜生长前，将s-HA圆盘用蔓越莓提取物/赋形剂对照处理5分钟，随后在生物膜生长12小时和24小时后，进行类似的暴露时间处理。然后对处理过的24小时龄生物膜进行产酸能力、代谢活性、胞外多糖（EPS）/微生物生物体积、结构组织和菌落形成单位（CFU）计数评估。与对照处理的生物膜相比，用500至1000μg/mL的蔓越莓提取物处理可使产酸能力和代谢活性显著降低（P<0.0001）。EPS的生物体积（P=0.003）和微生物生物膜成分（P=0.007）也显著降低。共聚焦生物膜图像的定性评估显示，蔓越莓提取物破坏了生物膜的结构架构。最后，与对照处理的生物膜相比，从蔓越莓处理的生物膜中回收的变形链球菌（P=0.006）和白色念珠菌（P=0.036）CFU显著减少。结论：在体外模型中，蔓越莓提取物抑制了双物种生物膜的致龋毒力特性。
