Programa de Pós-graduação em Doenças Infecciosas e Parasitárias, Universidade Federal de Mato Grosso do Sul - UFMS, Campo Grande, MS, Brasil.
Laboratório de Biologia Molecular do Carrapato, Departamento de Sanidade Animal, Empresa Brasileira de Pesquisa Agropecuária - Embrapa Gado de Corte, Campo Grande, MS, Brasil.
Rev Bras Parasitol Vet. 2020 Nov 20;29(4):e010820. doi: 10.1590/S1984-29612020097. eCollection 2020.
Neospora caninum is an obligate intracellular protozoan with canids (Canis domesticus, Canis lupus dingo, Canis latrans, Canis lupus) as its definitive hosts. The objective of this study was to detect anti-N. caninum antibodies in pregnant women seen at referral center for prenatal screening in the state of state Mato Grosso do Sul, Brazil. A total of 188 serum samples from pregnant women provided by the Instituto de Pesquisa, Ensino e Diagnósticos da APAE de Campo Grande (IPED/ APAE) were subjected to IFA test and western blot analysis. The samples were divided into three groups: 23/99 samples from the seropositive group for toxoplasmosis were positive for anti-N. caninum IgG antibodies, and 9/99 positive for IgM; in the HIV group, 7/33 were positive for IgG; and in the HIV+toxoplasmosis group, 13/56 were positive for IgG and two positive for IgM. The seropositivity for IgG was assessed by western blot by testing 43 IFA test positive samples using rNcSRS2 (Nc-p43) as antigen. The serological results of the present study suggest that exposure of these pregnant women to the parasite N. caninum and presence of IgM antibodies are indicative of recent infection. Further studies are needed to establish the possibility of active infection.
刚地弓形虫是一种专性细胞内原生动物，犬科动物（犬、狼、野犬、狼）为其终末宿主。本研究旨在检测巴西南马托格罗索州产前筛查转诊中心孕妇的抗刚地弓形虫抗体。共检测了来自 APAE 研究所、教育和诊断研究所（IPED/APAE）的 188 份孕妇血清样本，采用间接免疫荧光法（IFA）和免疫印迹法（WB）进行检测。将这些样本分为三组：23/99 份来自弓形虫血清阳性组的样本抗刚地弓形虫 IgG 抗体阳性，9/99 份抗 IgM 阳性；HIV 组中，7/33 份 IgG 阳性；在 HIV+弓形虫组中，13/56 份 IgG 阳性，2 份 IgM 阳性。通过使用 rNcSRS2（Nc-p43）作为抗原检测 43 份 IFA 试验阳性样本的 WB 来评估 IgG 的血清阳性率。本研究的血清学结果表明，这些孕妇接触刚地弓形虫和存在 IgM 抗体表明存在近期感染。需要进一步研究以确定是否存在活动性感染。
