Universidade Federal de Campina Grande - UFCG, Biological Sciences Academic Center, Patos, PB, Brazil.
Universidade Federal do Amazonas - UFAM, Health and Biotechnology Institute, Coari, AM, Brazil.
Braz Oral Res. 2020 Nov 23;35:e019. doi: 10.1590/1807-3107bor-2021.vol35.0019. eCollection 2020.
Matrix degradation is an important event in the progression, invasion and metastasis of malignant head and neck lesions. Imbalances, mutations and polymorphisms of MMPs and their inhibitors are observed in several cancer subtypes. The aim of this study was to evaluate the association of the MMP-7 gene promoter (181 A/G) and MMP-9 (-1562 C/T) polymorphisms in oral tongue squamous cell carcinoma (OTSCC). MMP-7 (rs11568818) and MMP-9 (rs3918242) single-nucleotide polymorphisms (SNPs) were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis in 71 cases of OTSCC. Normal tissue specimens were obtained from 60 healthy volunteers to serve as the control. The MMP-7 G allele and MMP-9 T allele were more frequent in the OTSCC group than the control group, but only when these two SNPs were taken together was a significant association found with the nodal metastasis of OTSCC (p < 0.001). Based on our results, SNPs in the promoter region of MMP-7 and MMP-9 appear to be associated with greater risk of developing OTSCC, and with a higher propensity to form metastatic tumors. In this respect, molecular studies investigating polymorphisms may be useful in predicting tumor behavior.
基质降解是恶性头颈部病变进展、侵袭和转移的一个重要事件。在几种癌症亚型中观察到 MMPs 及其抑制剂的失衡、突变和多态性。本研究旨在评估 MMP-7 基因启动子（181A/G）和 MMP-9（-1562C/T）多态性与口腔舌鳞状细胞癌（OTSCC）的相关性。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析，对 71 例 OTSCC 患者的 MMP-7（rs11568818）和 MMP-9（rs3918242）单核苷酸多态性（SNP）进行基因分型。从 60 名健康志愿者中获得正常组织标本作为对照。与对照组相比，OTSCC 组的 MMP-7 G 等位基因和 MMP-9 T 等位基因更为频繁，但只有当这两个 SNP 一起考虑时，才与 OTSCC 的淋巴结转移有显著相关性（p<0.001）。基于我们的结果，MMP-7 和 MMP-9 启动子区域的 SNP 似乎与 OTSCC 发病风险增加以及形成转移性肿瘤的倾向增加相关。在这方面，研究多态性的分子研究可能有助于预测肿瘤行为。
