基于三明治结构的抗体微阵列用于蛋白质检测。

Sandwich-Based Antibody Arrays for Protein Detection.

机构信息

RayBiotech Life, Peachtree Corners, GA, USA.

RayBiotech, Inc., Guangzhou, Guangdong, China.

出版信息

Methods Mol Biol. 2021;2237:1-10. doi: 10.1007/978-1-0716-1064-0_1.

DOI:10.1007/978-1-0716-1064-0_1

PMID:33237404

Abstract

Sandwich-based antibody arrays enable the detection of multiple proteins simultaneously, thus offering a time- and cost-effective alternative to single-plex platforms. The protein of interest is "sandwiched" between an antibody that captures it to the array and a second antibody that is used for detection. Here we describe a 1-day procedure to process samples, such as serum or cell lysates, with a quantitative sandwich-based antibody array on a glass substrate using fluorescence.

摘要

基于三明治夹心法的抗体芯片能够同时检测多种蛋白质，因此与单重平台相比，这种方法具有时间和成本效益高的优势。夹心法中，感兴趣的蛋白质被夹在能够捕获它的抗体和用于检测的第二抗体之间。在此，我们描述了一种在玻璃基质上使用荧光标记的基于三明治夹心法的抗体芯片，在一天内定量处理血清或细胞裂解液等样本的方法。