基于标记的抗体阵列的蛋白质全局检测。

Global Detection of Proteins by Label-Based Antibody Array.

机构信息

RayBiotech Life, Peachtree Corners, GA, USA.

Department of Gynaecologic Oncology, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, Guangdong, China.

出版信息

Methods Mol Biol. 2021;2237:45-53. doi: 10.1007/978-1-0716-1064-0_4.

DOI:10.1007/978-1-0716-1064-0_4

PMID:33237407

Abstract

Because of narrow availability of antibody pairs and potential cross-reactivity between antibodies, the development of sandwich-based antibody arrays which need a pair of antibodies for each target has been restricted to higher density resulting in limited proteomic breadth of detection. Label-based array is one way to overcome this obstacle by directly labeling all targets in samples with fluorescent dyes such as Cy3 and Cy5. The labeled samples are then applied on the antibody array chip composed of capture antibodies. In this chapter, we will introduce this technology including array production and sample detection assay.

摘要

由于抗体对的可用性有限，且抗体之间存在潜在的交叉反应，因此需要为每个目标开发一对抗体的基于夹心的抗体阵列的发展受到限制，导致检测的蛋白质组学范围有限。基于标记的阵列是克服这一障碍的一种方法，它可以通过用荧光染料（如 Cy3 和 Cy5）直接标记样品中的所有靶标来实现。然后将标记的样品应用于由捕获抗体组成的抗体阵列芯片上。在本章中，我们将介绍这项技术，包括阵列的制备和样品检测分析。