Iruzubieta Pablo, Cantarero Irene, Monzón Marta, Lahoz Manuel, Junquera Concepción
Department of Human Anatomy and Histology, Faculty of Medicine, University of Zaragoza, Zaragoza, Spain.
Institute for Health Research Aragón (IIS), Domingo Miral s/n, 50009, Zaragoza, Spain.
Cell Mol Neurobiol. 2022 Mar;42(2):473-481. doi: 10.1007/s10571-020-01017-8. Epub 2020 Nov 25.
Adult neurogenesis has been profusely studied in central nervous system. However, its presence in enteric nervous system remains elusive although it has been recently demonstrated in mice and intimately linked to glial cells. Moreover, primary cilium is an important organelle in central adult neurogenesis. In the present study, we analysed some parallelisms between central and enteric nervous system (ENS) in humans based on ultrastructural and immunohistochemical techniques. Thus, we described the presence of primary cilia in some subtypes of glial cells and Interstitial Cells of Cajal (ICCs) and we performed 3-D reconstructions to better characterise their features. Besides, we studied the expression of several adult neurogenesis-related proteins. Immature neuron markers were found in human ENS, supporting the existence of adult neurogenesis. However, only ICCs showed proliferation markers. Hence, we propose a new paradigm where ICCs would constitute the original neural stem cells which, through asymmetrical cell division, would generate the new-born neurons.
成人神经发生已在中枢神经系统中得到广泛研究。然而，尽管最近在小鼠中已证实其存在并与神经胶质细胞密切相关，但其在肠神经系统中的存在仍不明确。此外，初级纤毛是中枢成人神经发生中的重要细胞器。在本研究中，我们基于超微结构和免疫组织化学技术分析了人类中枢和肠神经系统（ENS）之间的一些相似之处。因此，我们描述了神经胶质细胞的某些亚型和 Cajal 间质细胞（ICC）中存在初级纤毛，并进行了三维重建以更好地表征其特征。此外，我们研究了几种与成人神经发生相关蛋白的表达。在人类 ENS 中发现了未成熟神经元标志物，支持成人神经发生的存在。然而，只有 ICC 显示出增殖标志物。因此，我们提出了一种新的范式，即 ICC 将构成原始神经干细胞，通过不对称细胞分裂产生新生神经元。
