D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology, St. Petersburg, Russia.
Bull Exp Biol Med. 2020 Nov;170(1):123-127. doi: 10.1007/s10517-020-05017-y. Epub 2020 Nov 25.
We studied the effect of microvesicles derived from cells of the NK-92 cell line on the formation of tube-like structures by endothelial cells of the ЕА.Hy926 cell line. Microvesicles were isolated by differential centrifugation and their size was controlled by granulometric analysis using dynamic light scattering method. The effect of microvesicles produced by NK cells on angiogenesis was evaluated by cultural methods. In the course of the research, a model of co-culturing of microvesicles and endothelial cells on extracellular matrix Matrigel was developed. It was found that microvesicles derived from NK-92 cells promoted elongation of tube-like structures formed by endothelial ЕА.Hy926 cells. Microvesicles produced by NK cells can modulate functional activity of endothelial cells by affecting their ability to form blood vessels.
我们研究了源自 NK-92 细胞系的细胞的微泡对内皮细胞 EA.Hy926 细胞系形成管状结构的影响。通过差速离心分离微泡，并使用动态光散射法通过粒度分析控制其大小。通过培养方法评估 NK 细胞产生的微泡对血管生成的影响。在研究过程中，开发了一种在细胞外基质 Matrigel 上共培养微泡和内皮细胞的模型。结果发现，源自 NK-92 细胞的微泡促进了内皮 EA.Hy926 细胞形成的管状结构的伸长。NK 细胞产生的微泡可以通过影响其形成血管的能力来调节内皮细胞的功能活性。
