LAMC, Laboratoire de l'Angiogenèse et du Microenvironnement des Cancers (Inserm U1029) F-33170 Pessac, France.
Université de Bordeaux, F-33170 Pessac, France.
Sci Adv. 2019 Jun 12;5(6):eaau6562. doi: 10.1126/sciadv.aau6562. eCollection 2019 Jun.
Most achievements to engineer blood vessels are based on multiple-step manipulations such as manual sheet rolling or sequential cell seeding followed by scaffold degradation. Here, we propose a one-step strategy using a microfluidic coextrusion device to produce mature functional blood vessels. A hollow alginate hydrogel tube is internally coated with extracellular matrix to direct the self-assembly of a mixture of endothelial cells (ECs) and smooth muscle cells (SMCs). The resulting vascular structure has the correct configuration of lumen, an inner lining of ECs, and outer sheath of SMCs. These "vesseloids" reach homeostasis within a day and exhibit the following properties expected for functional vessels (i) quiescence, (ii) perfusability, and (iii) contractility in response to vasoconstrictor agents. Together, these findings provide an original and simple strategy to generate functional artificial vessels and pave the way for further developments in vascular graft and tissue engineering and for deciphering the angiogenesis process.
大多数血管工程学成就都是基于多步操作，例如手动片状滚动或顺序细胞接种，然后进行支架降解。在这里，我们提出了一种使用微流控共挤出设备生产成熟的功能性血管的一步策略。中空的海藻酸盐水凝胶管内部涂有细胞外基质，以指导内皮细胞（EC）和平滑肌细胞（SMC）混合物的自组装。得到的血管结构具有正确的腔内腔、EC 内层和 SMC 外层。这些“血管样结构”在一天内达到内稳态，并表现出功能性血管的以下特性：（i）静止性、（ii）可灌注性和（iii）对血管收缩剂的收缩性。总之，这些发现为生成功能性人工血管提供了一种新颖而简单的策略，并为血管移植物和组织工程的进一步发展以及解析血管生成过程铺平了道路。
