Division of Immunology and Allergy, Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
Karolinska University Hospital, Stockholm, Sweden.
Methods Mol Biol. 2021;2236:9-17. doi: 10.1007/978-1-0716-1060-2_2.
While myeloid-derived suppressor cells (MDSCs) in humans and mice have been intensively investigated, there is limited knowledge of these cells in nonhuman primates (NHPs). NHPs serve as critical models for late-stage testing of several biomedical inventions before proceeding with clinical trials and it is therefore important to fully understand their immune compartments and similarities with humans. Here, using antihuman cross-reactive antibodies, we provide flow cytometric analysis protocols for identification of MDSCs in the blood of rhesus macaques, one of the major NHP species as experimental models. Discrepancies and similarities between rhesus and human MDSCs are discussed.
虽然人类和小鼠的髓系来源抑制细胞（MDSCs）已得到深入研究，但关于非人类灵长类动物（NHP）中的这些细胞的知识却有限。NHP 是几种生物医学发明在进行临床试验之前进行晚期测试的重要模型，因此充分了解它们的免疫成分以及与人类的相似之处非常重要。在这里，我们使用抗人交叉反应性抗体，提供了用于鉴定恒河猴血液中 MDSCs 的流式细胞术分析方案，恒河猴是实验模型中的主要 NHP 物种之一。讨论了恒河猴和人类 MDSCs 之间的差异和相似之处。
