Experimental and Translational Research, Department of Otorhinolaryngology, University Hospital Essen, West German Cancer Center, Essen, Germany.
Methods Mol Biol. 2021;2236:1-7. doi: 10.1007/978-1-0716-1060-2_1.
Myeloid-derived suppressor cells (MDSC) are a heterogeneous group of pathologically expanded myeloid cells with immunosuppressive activity. According to their phenotype, MDSC can be divided into three major subpopulations: early stage MDSC (e-MDSC), lacking myeloid lineage markers, monocytic MDSC (M-MDSC), and granulocytic MDSC (PMN-MDSC). Additionally, PMN-MDSC can be subdivided based on their activation and differentiation status, although it is not clear how this status contributes to immunosuppression and disease pathology. Here, we describe an immunophenotyping and gating strategy for the identification and isolation of MDSC subsets based on fluorescence-activated cell sorting. This method allows direct comparison of MDSC subsets in clinical settings.
髓系来源的抑制细胞（MDSC）是一群具有病态扩增和免疫抑制活性的异质性髓系细胞。根据其表型，MDSC 可分为三个主要亚群：早期 MDSC（e-MDSC），缺乏髓系谱系标记物；单核细胞来源的 MDSC（M-MDSC）；粒细胞来源的 MDSC（PMN-MDSC）。此外，PMN-MDSC 可以根据其激活和分化状态进一步细分，尽管其状态如何有助于免疫抑制和疾病发病机制尚不清楚。在这里，我们描述了一种基于流式细胞术的免疫表型和门控策略，用于鉴定和分离 MDSC 亚群。该方法允许在临床环境中直接比较 MDSC 亚群。
