Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany.
Institute for Virology, University Hospital, University Duisburg-Essen, Essen, Germany.
Methods Mol Biol. 2021;2236:189-201. doi: 10.1007/978-1-0716-1060-2_15.
Intravital imaging allows the visualization of fluorescently labeled structures like cells, blood flow, and pathogens in a living organism. Nowadays, numerous methods for imaging in several organs are available. In this chapter, we present a method for intravital 2-photon microscopy of the murine tibial bone marrow. It enables the observation of hematopoietic cells including cells of the innate and adaptive immune system under physiological conditions. Motility analyses within this complex environment led to insights into their migratory potential as well as their interactions with other cells or blood vessels.
活体成像可以在活体生物体内可视化荧光标记的结构，如细胞、血流和病原体。如今，有许多方法可用于对多个器官进行成像。在本章中，我们介绍了一种用于活体双光子显微镜检查的鼠胫骨骨髓方法。它能够在生理条件下观察造血细胞，包括先天和适应性免疫系统的细胞。在这个复杂的环境中进行的运动分析使我们深入了解它们的迁移潜力以及与其他细胞或血管的相互作用。
