Figueiredo Mariana Pires, Borrego-Sánchez Ana, García-Villén Fátima, Miele Dalila, Rossi Silvia, Sandri Giuseppina, Viseras César, Constantino Vera Regina Leopoldo
Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo-USP, Av. Prof. Lineu Prestes 748, São Paulo 05508-000, Brazil.
Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada-UGR, Campus of Cartuja s/n, 18071 Granada, Spain.
Pharmaceutics. 2020 Nov 23;12(11):1130. doi: 10.3390/pharmaceutics12111130.
This work presents the development of multifunctional therapeutic membranes based on a high-performance block copolymer scaffold formed by polyether (PE) and polyamide (PA) units (known as PEBA) and layered double hydroxide (LDH) biomaterials, with the aim to study their uses as wound dressings. Two LDH layer compositions were employed containing Mg or Zn, Fe and Al cations, intercalated with chloride anions, abbreviated as Mg-Cl or Zn-Cl, or intercalated with naproxenate (NAP) anions, abbreviated as Mg-NAP or Zn-NAP. Membranes were structurally and physically characterized, and the in vitro drug release kinetics and cytotoxicity assessed. PEBA-loading NaNAP salt particles were also prepared for comparison. Intercalated NAP anions improved LDH-polymer interaction, resulting in membranes with greater mechanical performance compared to the polymer only or to the membranes containing the Cl-LDHs. Drug release (in saline solution) was sustained for at least 8 h for all samples and release kinetics could be modulated: a slower, an intermediate and a faster NAP release were observed from membranes containing Zn-NAP, NaNAP and Mg-NAP particles, respectively. In general, cell viability was higher in the presence of Mg-LDH and the membranes presented improved performance in comparison with the powdered samples. PEBA containing Mg-NAP sample stood out among all membranes in all the evaluated aspects, thus being considered a great candidate for application as multifunctional therapeutic dressings.
这项工作展示了基于由聚醚（PE）和聚酰胺（PA）单元形成的高性能嵌段共聚物支架（称为PEBA）和层状双氢氧化物（LDH）生物材料的多功能治疗性膜的开发，旨在研究它们作为伤口敷料的用途。使用了两种LDH层组合物，分别含有Mg或Zn、Fe和Al阳离子，并插层有氯离子，简称为Mg-Cl或Zn-Cl，或者插层有萘普生盐（NAP）阴离子，简称为Mg-NAP或Zn-NAP。对膜进行了结构和物理表征，并评估了体外药物释放动力学和细胞毒性。还制备了负载PEBA的NaNAP盐颗粒用于比较。插层的NAP阴离子改善了LDH与聚合物的相互作用，导致与仅含聚合物或含Cl-LDHs的膜相比，膜具有更高的机械性能。所有样品在盐溶液中的药物释放持续至少8小时，并且释放动力学可以调节：分别从含有Zn-NAP、NaNAP和Mg-NAP颗粒的膜中观察到较慢、中等和较快的NAP释放。一般来说，在Mg-LDH存在下细胞活力更高，并且与粉末样品相比，膜表现出更好的性能。在所有评估方面，含Mg-NAP的PEBA样品在所有膜中脱颖而出，因此被认为是作为多功能治疗敷料应用的极佳候选者。
