环状 RNA0001859：COPD 和 AECOPD 的新型诊断和预后生物标志物。

CircRNA0001859, a new diagnostic and prognostic biomarkers for COPD and AECOPD.

机构信息

The First Affiliated Hospital, College of Medicine, Zhejiang University, NO.79, Qingchun Road, Hangzhou, 310003, China.

出版信息

BMC Pulm Med. 2020 Nov 25;20(1):311. doi: 10.1186/s12890-020-01333-1.

BACKGROUND

Dysregulation of circRNAs has been reported to be functionally associated with chronic obstructive pulmonary disease (COPD). The present investigation elucidated the potential role of CircRNA0001859 in regulating chronic obstructive pulmonary disease acute (COPD) and Acute Exacerbation of COPD (AECOPD).

METHODS

Mice model of COPD was established to screen and verify the dysregulated expression of CircRNA0001859. Fluorescence in situ hybridization (FISH) and quantitative real-time PCR (qRT-PCR) were carried out to detect the expression of CircRNA0001859. 38 stable COPD patients, 24 AECOPD patients, 57 COPD with lung cancer patients and 28 healthy person with age and sex matched to total patients were used for the present investigation.

RESULTS

circRNA0001859 was downregulated in the lung tissue of mice after the three kinds of treatments (Cigarette smoke (CS)/NK alone or CS + NNK) for inducing COPD. FISH assay verified the downregulation of circRNA0001859 both in the mice lung and human bronchial epithelial cell of COPD model. Furthermore,, the level of circRNA0001859 was also downregulated in the peripheral blood of COPD and lung cancer patients. CircRNA0001859 might act as a diagnostic and prognostic biomarker for the treatment of in COPD and AECOPD with Are under the receiver operating characteristic curve (ROC curve) (AUC) of 0.7433 and 0.8717, respectively.

CONCLUSION

We explored a novel circRNA0001859, which might act as a potential therapeutic biomarker for the treatment of COPD and AECOPD.

背景

circRNA 的失调已被报道与慢性阻塞性肺疾病（COPD）的功能相关。本研究阐明了 CircRNA0001859 在调节慢性阻塞性肺疾病急性（COPD）和 COPD 急性加重（AECOPD）中的潜在作用。

方法

建立 COPD 小鼠模型，筛选和验证 CircRNA0001859 的失调表达。采用荧光原位杂交（FISH）和实时定量 PCR（qRT-PCR）检测 CircRNA0001859 的表达。本研究共纳入 38 例稳定 COPD 患者、24 例 AECOPD 患者、57 例 COPD 合并肺癌患者和 28 例年龄和性别匹配的健康对照者。

结果

三种方法（香烟烟雾（CS）/自然杀伤细胞（NK）单独或 CS+NNK）诱导 COPD 后，小鼠肺组织中 circRNA0001859 下调。FISH 检测验证了 COPD 模型小鼠肺组织和人支气管上皮细胞中 circRNA0001859 的下调。此外，COPD 和肺癌患者外周血中 circRNA0001859 水平也下调。CircRNA0001859 可能作为 COPD 和 AECOPD 治疗的诊断和预后生物标志物，ROC 曲线（AUC）分别为 0.7433 和 0.8717。