Venkata Bala P, Polzin Robert, Wilkes Rebecca, Fearn Armahni, Blumenthal Dylan, Rohrbough Sara, Taylor Nigel J
Donald Danforth Plant Science Center, St. Louis, MO, United States.
Front Plant Sci. 2020 Nov 10;11:515078. doi: 10.3389/fpls.2020.515078. eCollection 2020.
Heterologous overexpression of Arabidopsis cellulase 1 () results in enhanced yield, early maturity, and increased biomass in dicotyledonous species like poplar and eucalyptus but has not been demonstrated in monocots. We produced transgenic accession A10.1 plants overexpressing a monocotyledonous codon optimized (. Agronomic characterization of the transgenic events showed that heterologous overexpression of caused enhanced grain yield, shoot biomass, and accelerated maturation rate in the model grass species under growth chamber conditions. The agronomic trait differences observed were consistent with previous reports in dicots but are here described in a monocot species and associated with increased seed yield. Overexpression of in was shown to increase the number of panicles and seeds by 24-30%, enhance overall grain yield by up to 26%, and lead to a shoot dry biomass increase of 16-19%. Overexpression also reduced time to plant maturation and senescence by 12.5%. Our findings in suggest that manipulation of has potential for developing early-maturing and higher-yielding monocotyledonous biomass crops suitable for climate-smart agriculture.
拟南芥纤维素酶1（）的异源过表达导致杨树和桉树等双子叶植物产量提高、早熟且生物量增加，但在单子叶植物中尚未得到证实。我们培育了过表达单子叶植物密码子优化的（）转基因A10.1植株。对转基因事件的农艺学特征分析表明，在生长室条件下，在模式禾本科植物中异源过表达导致籽粒产量、地上部生物量提高，成熟速率加快。观察到的农艺性状差异与之前双子叶植物的报道一致，但这里是在单子叶植物中描述的，并且与种子产量增加有关。在中过表达显示，穗数和种子数增加24 - 30%，总籽粒产量提高多达26%，地上部干生物量增加16 - 19%。过表达还使植株成熟和衰老时间缩短了12.5%。我们在中的研究结果表明，操纵有潜力培育适合气候智能型农业的早熟、高产单子叶生物量作物。
