Weraduwage Sarathi M, Chen Jin, Anozie Fransisca C, Morales Alejandro, Weise Sean E, Sharkey Thomas D
Department of Biochemistry and Molecular Biology, Michigan State University East Lansing, MI, USA.
Department of Energy Plant Research Laboratory, Michigan State University East Lansing, MI, USA ; Department of Computer Science and Engineering, Michigan State University East Lansing, MI, USA.
Front Plant Sci. 2015 Apr 9;6:167. doi: 10.3389/fpls.2015.00167. eCollection 2015.
Leaf area growth determines the light interception capacity of a crop and is often used as a surrogate for plant growth in high-throughput phenotyping systems. The relationship between leaf area growth and growth in terms of mass will depend on how carbon is partitioned among new leaf area, leaf mass, root mass, reproduction, and respiration. A model of leaf area growth in terms of photosynthetic rate and carbon partitioning to different plant organs was developed and tested with Arabidopsis thaliana L. Heynh. ecotype Columbia (Col-0) and a mutant line, gigantea-2 (gi-2), which develops very large rosettes. Data obtained from growth analysis and gas exchange measurements was used to train a genetic programming algorithm to parameterize and test the above model. The relationship between leaf area and plant biomass was found to be non-linear and variable depending on carbon partitioning. The model output was sensitive to the rate of photosynthesis but more sensitive to the amount of carbon partitioned to growing thicker leaves. The large rosette size of gi-2 relative to that of Col-0 resulted from relatively small differences in partitioning to new leaf area vs. leaf thickness.
叶面积增长决定了作物的光截获能力，在高通量表型分析系统中常被用作植物生长的替代指标。叶面积增长与质量增长之间的关系将取决于碳在新叶面积、叶质量、根质量、繁殖和呼吸作用之间的分配方式。建立了一个基于光合速率和碳分配到不同植物器官的叶面积增长模型，并用拟南芥哥伦比亚生态型（Col-0）和一个莲座叶非常大的突变系巨叶2（gi-2）进行了测试。从生长分析和气体交换测量中获得的数据用于训练遗传编程算法，以对上述模型进行参数化和测试。发现叶面积与植物生物量之间的关系是非线性的，并且根据碳分配情况而变化。模型输出对光合速率敏感，但对分配到生长更厚叶片的碳量更敏感。gi-2相对于Col-0的大莲座叶大小是由于分配到新叶面积与叶厚度的差异相对较小所致。
