Landínez-García Ricardo M, Narváez Juan Carlos, Márquez Edna J
Facultad de Ciencias, Escuela de Biociencias, Laboratorio de Biología Molecular y Celular, Universidad Nacional de Colombia-Sede Medellín, Medellín, Colombia.
Grupo de Biodiversidad y Ecología Aplicada, Laboratorio de Genética Molecular, Universidad del Magdalena, Santa Marta, Magdalena, Colombia.
PeerJ. 2020 Nov 11;8:e10327. doi: 10.7717/peerj.10327. eCollection 2020.
is a freshwater fish endemic to the Colombian Magdalena-Cauca and Caribbean hydrographic basins. The genetic structure patterns of populations of different members of and the historic restocking of its depleted natural populations suggest that exhibits genetic stocks that coexist and co-migrate throughout the rivers Magdalena, Cauca, Cesar, Sinú and Atrato. To test this hypothesis and explore the levels of genetic diversity and population demography of 725 samples of . from the studied rivers, we developed a set of 11 species-specific microsatellite loci using next-generation sequencing, bioinformatics, and experimental tests of the levels of diversity of the microsatellite loci. The results evidenced that exhibits high genetic diversity, significant inbreeding coefficient ranging from 0.162 to 0.202, and signs of erosion of the genetic pool. Additionally, the population genetic structure constitutes a mixture of genetic stocks heterogeneously distributed along the studied rivers, and moreover, a highly divergent genetic stock was detected in Chucurí, Puerto Berrío and Palagua that may result from restocking practices. This study provides molecular tools and a wide framework regarding the genetic diversity and structure of , which is crucial to complement its baseline information, diagnosis and monitoring of populations, and to support the implementation of adequate regulation, management, and conservation policies.
是一种原产于哥伦比亚马格达莱纳 - 考卡和加勒比水文流域的淡水鱼。不同成员种群的遗传结构模式及其枯竭自然种群的历史补充情况表明，在马格达莱纳河、考卡河、塞萨尔河、锡努河和阿特拉托河的整个流域中，存在着共存和共同迁移的遗传种群。为了验证这一假设并探索来自研究河流的725个样本的遗传多样性水平和种群统计学，我们利用下一代测序、生物信息学以及微卫星位点多样性水平的实验测试，开发了一组11个物种特异性微卫星位点。结果表明，该鱼表现出高遗传多样性、显著的近交系数在0.162至0.202之间，以及遗传库受到侵蚀的迹象。此外，种群遗传结构构成了沿研究河流异质分布的遗传种群的混合体，而且在丘库里、贝里奥港和帕拉瓜发现了一个高度分化的遗传种群，这可能是由于补充放流实践导致的。本研究提供了关于该鱼遗传多样性和结构的分子工具及广泛框架，这对于补充其基线信息、种群诊断和监测以及支持实施适当的监管、管理和保护政策至关重要。
