Bryant D H, Kay A B
Clin Allergy. 1977 May;7(3):211-7. doi: 10.1111/j.1365-2222.1977.tb01445.x.
The numbers of eosinophils recruited locally to abraded human skin were measured in eight atopic and eight non-atopic volunteers, at time intervals over 24 hr, following the application of an ECF-A tetrapeptide (Val-Gly-Ser-Glu) or histamine. In all subjects the higher doses of the peptide (10(-4) and 10(-6) mol/l) or histamine (10(-3) and 10(-5) mol/l) produced significantly greater counts than the Tyrode's diluent alone. The counts produced with the lowest dose of peptide (10(-8) mol/l) or histamine (10(-7) mol/l) were not significantly different from the control. The peptide or histamine evoked a greater local eosinophilia in the atopics than the non-atopics. This effect was probably independent of the peripheral blood eosinophil counts since at the time of study the numbers of circulating eosinophils between the two groups were not significantly different. In the atopics, histamine in doses of 10(-3) and 10(-5) mol/l were required to give the same eosinophil response as that obtained with 10(-4) and 10(-6) mol/l of the peptide, respectively. It is suggested that the relative paucity of eosinophils recruited by locally applied ECF-A peptide or histamine, when compared to antigen-induced eosinophilia, is due either to an inability to mimic the events associated with the release of these mediators from mast-cells or the involvement of other pharmacological agents.
在8名特应性志愿者和8名非特应性志愿者中，于24小时内的不同时间间隔，测量了在应用嗜酸性粒细胞趋化因子A四肽（缬氨酸 - 甘氨酸 - 丝氨酸 - 谷氨酸）或组胺后，局部募集到磨损的人体皮肤处的嗜酸性粒细胞数量。在所有受试者中，较高剂量的肽（10⁻⁴和10⁻⁶摩尔/升）或组胺（10⁻³和10⁻⁵摩尔/升）产生的计数显著高于单独使用台氏稀释液。最低剂量的肽（10⁻⁸摩尔/升）或组胺（10⁻⁷摩尔/升）产生的计数与对照组无显著差异。与非特应性受试者相比，肽或组胺在特应性受试者中引起了更大的局部嗜酸性粒细胞增多。这种效应可能与外周血嗜酸性粒细胞计数无关，因为在研究时两组之间循环嗜酸性粒细胞的数量无显著差异。在特应性受试者中，分别需要10⁻³和10⁻⁵摩尔/升的组胺才能产生与10⁻⁴和10⁻⁶摩尔/升的肽相同嗜酸性粒细胞反应。有人提出，与抗原诱导的嗜酸性粒细胞增多相比，局部应用嗜酸性粒细胞趋化因子A肽或组胺募集的嗜酸性粒细胞相对较少，这要么是由于无法模拟与这些介质从肥大细胞释放相关联的事件，要么是由于其他药理剂的参与。
