Abdel-Monem Nihad M, El-Saadani Mohammad A, Daba Ayman S, Saleh Samar R, Aleem Eiman
Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, Egypt.
RUSH University Medical Center, Chicago, USA.
Biochem Biophys Rep. 2020 Nov 12;24:100852. doi: 10.1016/j.bbrep.2020.100852. eCollection 2020 Dec.
Liver damage involves oxidative stress and a progression from chronic hepatitis to hepatocellular carcinoma (HCC). The increased incidence of liver disease in Egypt and other countries in the last decade, coupled with poor prognosis, justify the critical need to introduce alternative chemopreventive agents that may protect against liver damage. The aim of this study was to evaluate the efficacy of exopolysaccharide-peptide (PSP) complex extracted from as a hepatoprotective agent against diethylnitrosamine (DEN)/carbon tetrachloride (CCL)-induced hepatocellular damage in rats. The levels of liver injury markers (ALT, AST and ALP) were substantially increased following DEN/CCl treatment. DEN/CCl - induced oxidative stress was confirmed by elevated levels of lipid peroxidation and decreased levels of superoxide dismutase, glutathione-S-transferase, and reduced glutathione. PSP reversed these alterations in the liver and serum, and provided protection evidenced by reversal of histopathological changes in the liver. The present study demonstrated that PSP extract from exhibited hepatoprotective and antioxidant effects against DEN/CCl-induced hepatocellular damage in rats. Given the high prevalence of HCV-related liver damage in Egypt, our results suggest further clinical evaluation of extracts and their potential hepatoprotective effects in patients with liver disease.
肝损伤涉及氧化应激以及从慢性肝炎到肝细胞癌（HCC）的进展。在过去十年中，埃及和其他国家肝病发病率的上升，再加上预后不良，使得引入可能预防肝损伤的替代化学预防剂成为迫切需求。本研究的目的是评估从[具体来源未给出]提取的胞外多糖-肽（PSP）复合物作为一种肝保护剂对二乙基亚硝胺（DEN）/四氯化碳（CCl）诱导的大鼠肝细胞损伤的疗效。在DEN/CCl处理后，肝损伤标志物（ALT、AST和ALP）水平大幅升高。脂质过氧化水平升高以及超氧化物歧化酶、谷胱甘肽-S-转移酶和还原型谷胱甘肽水平降低证实了DEN/CCl诱导的氧化应激。PSP逆转了肝脏和血清中的这些改变，并通过肝脏组织病理学变化的逆转提供了保护作用。本研究表明，从[具体来源未给出]提取的PSP提取物对DEN/CCl诱导的大鼠肝细胞损伤具有肝保护和抗氧化作用。鉴于埃及丙型肝炎病毒相关肝损伤的高患病率，我们的结果表明应进一步对[具体来源未给出]提取物及其对肝病患者潜在的肝保护作用进行临床评估。
