School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, PR China.
Calcium and Carbide Factory, Jilin Petrochemical Company, Jilin 132022, PR China.
Int J Biol Macromol. 2019 Jun 15;131:1-9. doi: 10.1016/j.ijbiomac.2019.03.043. Epub 2019 Mar 7.
This study was conducted to evaluate the hepatoprotective effect of polysaccharides from Pleurotus ostreatus (POP) on carbon tetrachloride-induced acute liver injury (ALI) in rats. The hepatoprotective effect of POP against ALI was reflected by the decreased alanine transaminase and aspartate transaminase levels in blood, increased superoxide dismutase, catalase, and glutathione peroxidase levels, and decreased malondialdehyde levels in blood and liver. TUNEL and immunohistochemistry analyses showed that TUNEL apoptosis cell rate and Bax, cytochrome C, caspase-3, caspase-9, and apoptosis-inducing factor expression levels were obviously decreased, whereas Bcl-2 expression levels obviously increased after POP treatment. A total of 11 metabolites belonging to energy, amino acid, fatty acid, and gut bacteria metabolism were identified as biomarkers by using metabolite analysis of liver homogenate based on ultra-performance liquid chromatography-tandem mass spectrometry. POP exhibited hepatoprotective effects on CCl-induced ALI, and the underlying mechanism is correlated with antioxidants that regulate metabolic pathway disorders and alleviate liver mitochondria apoptosis.
本研究旨在评估糙皮侧耳多糖（POP）对四氯化碳诱导的大鼠急性肝损伤（ALI）的肝保护作用。POP 对 ALI 的肝保护作用体现在其降低了血液中丙氨酸转氨酶和天冬氨酸转氨酶的水平，增加了超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的水平，以及降低了血液和肝脏中丙二醛的水平。TUNEL 和免疫组织化学分析表明，POP 处理后 TUNEL 凋亡细胞率和 Bax、细胞色素 C、caspase-3、caspase-9 和凋亡诱导因子的表达水平明显降低，而 Bcl-2 的表达水平明显增加。通过基于超高效液相色谱-串联质谱的肝匀浆代谢物分析，共鉴定出 11 种属于能量、氨基酸、脂肪酸和肠道细菌代谢的代谢物作为生物标志物。POP 对 CCl 诱导的 ALI 具有肝保护作用，其作用机制与抗氧化剂有关，抗氧化剂可调节代谢途径紊乱并减轻肝线粒体凋亡。
