来自植物内生真菌叶点霉属Phyllosticta sp. WGHL2的抗真菌杂萜和二氧杂环戊酮衍生物
Antifungal meroterpenes and dioxolanone derivatives from plant-associated endophytic fungus Phyllosticta sp. WGHL2.
作者信息
Yan Wei, Zhao Shuangshuang, Gu Chenguang, Tian Kailin, Wang Zhiyang, Liu Fang, Ye Yonghao
机构信息
College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, PR China; Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, PR China.
College of Science, Nanjing Agricultural University, Nanjing 210095, PR China.
出版信息
Fitoterapia. 2021 Jan;148:104778. doi: 10.1016/j.fitote.2020.104778. Epub 2020 Nov 23.
Four new meroterpenes named as guignardones U-X (1-4), along with eleven known meroterpenes (5-15) and three known dioxolanone derivatives (16-18), were obtained from the endophytic fungus Phyllosticta sp. WGHL2. The structural elucidation was conducted by HRESIMS, NMR, single crystal X-ray diffraction, along with ECD calculations and comparison. In antifungal tests, compound 16 possessed broad-spectrum antifungal activities against Rhizoctonia solani, Fusarium graminearum and Botrytis cinerea with inhibition ratio of 48.43%, 40.98%, and 49.53% at 50 μg/mL, respectively. Moreover, compound 16 showed moderate protective effect against B. cinerea in vivo at 200 μg/mL and exhibited effective inhibition on the spore germination of B. cinerea.
从内生真菌叶点霉属Phyllosticta sp. WGHL2中获得了四种新的杂萜，命名为吉尼亚酮U-X（1-4），以及十一种已知杂萜（5-15）和三种已知的二氧戊环酮衍生物（16-18）。通过高分辨电喷雾电离质谱（HRESIMS）、核磁共振（NMR）、单晶X射线衍射以及电子圆二色光谱（ECD）计算和比较进行结构解析。在抗真菌试验中，化合物16对立枯丝核菌、禾谷镰刀菌和灰葡萄孢具有广谱抗真菌活性，在50 μg/mL时抑制率分别为48.43%、40.98%和49.53%。此外，化合物16在200 μg/mL时对灰葡萄孢在体内表现出中等保护作用，并对灰葡萄孢的孢子萌发具有有效抑制作用。
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