Reyes Castillo Nicolas, Rojas López-Menchero Jorge, Pacheco Useche William A, Díaz Carmen E, Andres M Fe, González-Coloma Azucena
Instituto de Ciencias Agrarias (ICA-CSIC), Serrano 115Dpdo, 28006, Madrid, Spain.
Institute for Insect Biotechnology With Focus On Natural Product Research, Justus-Liebig-University Giessen, Ohlebergsweg 12, 35392, Giessen, Germany.
Sci Rep. 2025 Mar 7;15(1):7989. doi: 10.1038/s41598-025-92626-0.
In the current work, the production of dioxolanone and meroterpene type secondary metabolites under different fermentation conditions by the endophytic fungus Phyllosticta capitalensis (isolate YCC4) isolated from the leaves of the endemic plant Persea indica has been evaluated. Different techniques, such as microparticle-enhanced culture (MPEC: bentonite, talcum powder) and surface adhesion fermentation (SAF: metallic mesh and glass wool) were applied to the culture medium. Ethyl acetate extracts from different fermentations of the isolate YCC4 were analyzed for their content and revealed the following dioxolanone derivatives: metguignardic acid (1), guignardianone C (2), ethyl guignardate (3), guignardianone D (4) and phenguignardic acid methyl ester (5), and meroterpenes: guignardone A (6) and B (7), guignarenone C (8), guignarenone B (9) and guignardone I (10). Additionally, the biological activity of extracts was tested against the aphid Myzus persicae and the root-knot nematode Meloidogyne javanica. Our study revealed significant variations in the production of the target metabolites as well as notable differences in biopesticide activity influenced by the presence of inert supports. Overall, the findings indicate that glass wool (GW) is a high-performance material to improve the production of dioxolanone derivatives. These findings underscore the significance of innovative cultivation methods such MPEC and SAF as drivers to produce valuable secondary metabolites from fungal organisms.
在当前工作中，对从地方特有植物鳄梨叶中分离得到的内生真菌首都叶点霉（菌株YCC4）在不同发酵条件下二氧戊环酮和杂萜类次生代谢产物的产生情况进行了评估。将不同技术，如微粒增强培养（MPEC：膨润土、滑石粉）和表面附着发酵（SAF：金属网和玻璃棉）应用于培养基。对菌株YCC4不同发酵产物的乙酸乙酯提取物进行含量分析，发现了以下二氧戊环酮衍生物：甲基吉尼亚酸（1）、吉尼亚酮C（2）、吉尼亚酸乙酯（3）、吉尼亚酮D（4）和苯基吉尼亚酸甲酯（5），以及杂萜类化合物：吉尼亚酮A（6）和B（7）、吉尼亚烯酮C（8）、吉尼亚烯酮B（9）和吉尼亚酮I（10）。此外，还测试了提取物对蚜虫桃蚜和根结线虫爪哇根结线虫的生物活性。我们的研究表明，目标代谢产物的产生存在显著差异，并且受惰性载体的影响，生物农药活性也存在明显差异。总体而言，研究结果表明玻璃棉（GW）是提高二氧戊环酮衍生物产量的高性能材料。这些发现强调了诸如MPEC和SAF等创新培养方法作为从真菌生物体中生产有价值次生代谢产物的驱动因素的重要性。
