Msx1 与 Runx1 合作抑制成肌细胞分化。

Msx1 cooperates with Runx1 for inhibiting myoblast differentiation.

机构信息

School of Life Sciences, Fudan University, Shanghai, 200438, China.

出版信息

Protein Expr Purif. 2021 Mar;179:105797. doi: 10.1016/j.pep.2020.105797. Epub 2020 Nov 24.

DOI:10.1016/j.pep.2020.105797

Abstract

Myogenesis is an important and complicated biological process, especially during the process of embryonic development. The homeoprotein Msx1 is a crucial transcriptional repressor of myogenesis and maintains myogenic precursor cells in an undifferentiated, proliferative state. However, the molecular mechanism through which Msx1 coordinates myogenesis remains to be elucidated. Here, we determine the interacting partner proteins of Msx1 in myoblast cells by a proteomic screening method. Msx1 is found to interact with 55 proteins, among which our data demonstrate that the cooperation of Runt-related transcription factor 1 (Runx1) with Msx1 is required for myoblast cell differentiation. Our findings provide important insights into the mechanistic roles of Msx1 in myoblast cell differentiation, and lays foundation for the myogenic differentiation process.

摘要

肌发生是一个重要且复杂的生物学过程，特别是在胚胎发育过程中。同源盒蛋白 Msx1 是肌发生的关键转录抑制因子，它使成肌前体细胞保持在未分化、增殖的状态。然而，Msx1 协调肌发生的分子机制仍有待阐明。在这里，我们通过蛋白质组筛选方法确定了 Msx1 在成肌细胞中的相互作用蛋白。结果发现 Msx1 与 55 种蛋白质相互作用，其中我们的数据表明，Runx1（ Runt 相关转录因子 1）与 Msx1 的合作对于成肌细胞的分化是必需的。我们的发现为 Msx1 在成肌细胞分化中的作用机制提供了重要的见解，并为成肌分化过程奠定了基础。