全球定量蛋白质组学分析揭示成肌细胞分化过程中Msx1和Msx2的下游信号网络。
Global Quantitative Proteomics Analysis Reveals the Downstream Signaling Networks of Msx1 and Msx2 in Myoblast Differentiation.
作者信息
Zhou Guoqiang, Ma Shuangping, Yang Ming, Yang Yenan
机构信息
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200438 China.
出版信息
Phenomics. 2022 Mar 14;2(3):201-210. doi: 10.1007/s43657-022-00049-y. eCollection 2022 Jun.
UNLABELLED
The msh homeobox 1 (Msx1) and msh homeobox 2 (Msx2) coordinate in myoblast differentiation and also contribute to muscle defects if altered during development. Deciphering the downstream signaling networks of Msx1 and Msx2 in myoblast differentiation will help us to understand the molecular events that contribute to muscle defects. Here, the proteomics characteristics in Msx1- and Msx2-mediated myoblast differentiation was evaluated using isobaric tags for the relative and absolute quantification labeling technique (iTRAQ). The downstream regulatory proteins of Msx1- and Msx2-mediated differentiation were identified. Bioinformatics analysis revealed that these proteins were primarily associated with xenobiotic metabolism by cytochrome P450, fatty acid degradation, glycolysis/gluconeogenesis, arginine and proline metabolism, and apoptosis. In addition, our data show Acta1 was probably a core of the downstream regulatory networks of Msx1 and Msx2 in myoblast differentiation.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s43657-022-00049-y.
未标注
肌肉特异性同源盒蛋白1（Msx1）和肌肉特异性同源盒蛋白2（Msx2）在成肌细胞分化过程中协同作用，并且如果在发育过程中发生改变也会导致肌肉缺陷。解析Msx1和Msx2在成肌细胞分化中的下游信号网络将有助于我们了解导致肌肉缺陷的分子事件。在此，使用相对和绝对定量同位素标记技术（iTRAQ）评估Msx1和Msx2介导的成肌细胞分化中的蛋白质组学特征。鉴定了Msx1和Msx2介导的分化的下游调节蛋白。生物信息学分析表明，这些蛋白质主要与细胞色素P450介导的异源生物代谢、脂肪酸降解、糖酵解/糖异生、精氨酸和脯氨酸代谢以及细胞凋亡有关。此外，我们的数据表明，肌动蛋白α1（Acta1）可能是Msx1和Msx2在成肌细胞分化中的下游调节网络的核心。
补充信息
在线版本包含可在10.1007/s43657-022-00049-y获取的补充材料。
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