Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), Alexandria, Egypt.
Prep Biochem Biotechnol. 2021;51(7):629-641. doi: 10.1080/10826068.2020.1848866. Epub 2020 Nov 27.
Lactoperoxidase is a glycosylated protein with a molecular mass of 78 kDa, which being excreted in several mammalian secretions. Lactoperoxidase is included in many biological processes and well-known to have biocidal actions, attending as active antibiotics and antiviral agents. This wide-spectrum of biocidal activities mediates via a definite inhibitory system named lactoperoxidase system which acts a potent role in the innate immune response since its activity is not restricted by the antimicrobial effect, but might act a significant role in the hydrolysis of many toxins like aflatoxin. Hence with the current progresses in technology, nanoparticles can offer chances as an active candidate that might be utilized for stabilizing and potentiating the activity of LPO for use in several applications. Due to the variability functions of LPO, this enzyme considers an active target to be encapsulated or coated to NPs for developing novel nanocombinations with controlled surface characteristics. The development of approaches which might enhance conformational stabilization for several weeks of LPO via nanoformulation could improve the biopharmaceutical applicability of this bioactive ingredient. Nanoformulation of LPO enhances novel functions that can be useful in many biotechnological applications like food industry, cosmetic and pharmaceutical applications or to deliver and encapsulate bioactive components.
乳过氧化物酶是一种糖基化蛋白，分子量为 78kDa，存在于多种哺乳动物的分泌物中。乳过氧化物酶参与许多生物过程，具有杀菌作用，是一种有效的抗生素和抗病毒药物。这种广谱杀菌活性是通过一种被称为乳过氧化物酶系统的特定抑制系统介导的，由于其活性不受抗菌作用的限制，因此在先天免疫反应中起着重要作用，其活性可能在许多毒素（如黄曲霉毒素）的水解中发挥重要作用。因此，随着技术的不断进步，纳米颗粒可以作为一种有前途的活性候选物，用于稳定和增强 LPO 的活性，以应用于多种应用。由于 LPO 的功能多样，这种酶被认为是一种活性靶点，可以被包封或涂覆到纳米颗粒上，以开发具有可控表面特性的新型纳米复合物。通过纳米制剂，可能会增强 LPO 构象稳定性数周，从而提高这种生物活性成分的生物制药适用性。LPO 的纳米制剂增强了其新功能，可用于食品工业、化妆品和制药应用等许多生物技术应用，或用于输送和包封生物活性成分。
