Sheikh Ishfaq Ahmad, Yasir Muhammad, Khan Imran, Khan Sher Bahadur, Azum Naveed, Jiffri Essam Hussain, Kamal Mohammad Amjad, Ashraf Ghulam Md, Beg Mohd Amin
King Fahd Medical Research Center, King Abdulaziz University,Jeddah,Kingdom of Saudi Arabia.
Department of Chemistry,Faculty of Science,Center of Excellence for Advanced Materials Research, King Abdulaziz University,Jeddah,Kingdom of Saudi Arabia.
J Dairy Res. 2018 Nov;85(4):460-464. doi: 10.1017/S0022029918000730. Epub 2018 Aug 23.
Lactoperoxidase (LPO) is an antimicrobial protein present in milk that plays an important role in natural defence mechanisms during neonatal and adult life. The antimicrobial activity of LPO has been commercially adapted for increasing the shelf life of dairy products. Immobilization of LPO on silver nanoparticles (AgNPs) is a promising way to enhance the antimicrobial activity of LPO. In the current study, LPO was immobilized on AgNPs to form LPO/AgNP conjugate. The immobilized LPO/AgNP conjugate was characterized by various biophysical techniques. The enhanced antibacterial activity of the conjugate was tested against E. coli in culture at 2 h intervals for 10 h. The results showed successful synthesis of spherical AgNPs. LPO was immobilized on AgNPs with agglomerate sizes averaging approximately 50 nm. The immobilized conjugate exhibited stronger antibacterial activity against E. coli in comparison to free LPO. This study may help in increasing the efficiency of lactoperoxidase system and will assist in identifying novel avenues to enhance the stability and antimicrobial function of LPO system in dairy and other industries.
乳过氧化物酶（LPO）是一种存在于牛奶中的抗菌蛋白，在新生儿和成年期的天然防御机制中发挥着重要作用。LPO的抗菌活性已被商业应用于延长乳制品的保质期。将LPO固定在银纳米颗粒（AgNPs）上是增强LPO抗菌活性的一种有前景的方法。在当前研究中，LPO被固定在AgNPs上以形成LPO/AgNP共轭物。通过各种生物物理技术对固定化的LPO/AgNP共轭物进行了表征。在培养物中，以2小时间隔对共轭物针对大肠杆菌的增强抗菌活性进行了10小时的测试。结果表明成功合成了球形AgNPs。LPO被固定在平均团聚尺寸约为50nm的AgNPs上。与游离LPO相比，固定化共轭物对大肠杆菌表现出更强的抗菌活性。这项研究可能有助于提高乳过氧化物酶系统的效率，并将有助于确定增强乳制品及其他行业中LPO系统稳定性和抗菌功能的新途径。
