Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Reprod Fertil Dev. 2020 Dec;32(18):1338-1349. doi: 10.1071/RD20107.
The aim of this study was to investigate the effect of calorie restriction (CR) during pregnancy in mice on metabolism and ovarian function in the offspring. Pregnant female mice were divided into two groups, a control group and a CR group (n=7 in each). Mice in the CR group were fed 50% of the amount consumed by control females from Day 10 of gestation until delivery. After weaning, the offspring received diet ad libitum until 3 months of age, when ovaries were collected. Ovaries were serially cut and every sixth section was used for follicle counting. Female offspring from CR dams tended to have increased bodyweight compared with offspring from control females (P=0.08). Interestingly, fewer primordial follicles (60% reduction; P=0.001), transitional follicles (P=0.0006) and total follicles (P=0.006) were observed in offspring from CR mothers. The number of primary, secondary and tertiary follicles did not differ between the groups (P>0.05). The CR offspring had fewer DNA double-strand breaks in primary follicle oocytes (P=0.03). In summary, CR during the second half of gestation decreased primordial ovarian follicle reserve in female offspring. These findings suggest that undernutrition during the second half of gestation may decrease the reproductive lifespan of female offspring.
本研究旨在探讨孕期限制卡路里摄入（CR）对小鼠后代代谢和卵巢功能的影响。将怀孕的雌性小鼠分为两组，对照组和 CR 组（每组 n=7）。CR 组的小鼠从妊娠第 10 天开始至分娩，其进食量为对照组雌鼠的 50%。断奶后，后代可自由进食，直至 3 月龄时采集卵巢。将卵巢连续切片，每 6 个切片用于卵泡计数。与对照组的后代相比，CR 组母鼠的雌性后代体重有增加的趋势（P=0.08）。有趣的是，CR 组母鼠的后代原始卵泡（减少 60%；P=0.001）、过渡卵泡（P=0.0006）和总卵泡（P=0.006）数量减少。两组间初级、次级和三级卵泡数量无差异（P>0.05）。CR 组后代的初级卵泡卵母细胞中 DNA 双链断裂较少（P=0.03）。总之，妊娠后半期的 CR 减少了雌性后代的原始卵巢卵泡储备。这些发现表明，妊娠后半期的营养不良可能会降低雌性后代的生殖寿命。
