Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Unit of Reproductive and Developmental Biology, Faculty of Science of Tunis, University of Tunis El Manar, Tunis, Tunisia.
J Ovarian Res. 2017 Dec 28;10(1):77. doi: 10.1186/s13048-017-0372-x.
Food restriction during pregnancy can influence the health of the offspring during the adulthood. The aim of the present study was to examine the effect of maternal food restriction (MFR) on the reproductive performance in female rat offspring from the first (FR1) and second (FR2) generations.
Adult virgin Wistar female rats were given free access to tap water and were fed ad libitum on standard rodent chow, were mated with virgin adult males, and then were randomly divided into two groups: controls (that was fed ad libitum ) and food-restricted group (FR, that was given only 50% of ad libitum food throughout gestation). Their first (FR1) and the second (FR2) generation of offspring were fed ad libitum and sacrificed before puberty and at adulthood. Their ovaries were removed and their histology evaluated by estimating the number of follicles (total and at various stages of folliculogenesis), and the presence of multi-nuclei oocytes and multi-oocyte follicles.
Total number of ovarian follicles was lower in FR1 females at week 4 in comparison with controls, while it was not different in FR2 females vs.
The number of the primordial follicle was lower in FR1 and FR2 females vs. controls at both week 4 and at week 8. When compared to the controls, the follicles containing multi-nuclei oocytes were more frequent in ovaries from FR1 and FR2 females at week 4, and higher and lower respectively in ovaries form FR1 and FR2 females at week 8.
MFR affects ovarian histology by inducing the development of abnormal follicles in the ovaries in first and second generation offspring. This finding could influence the ovarian function resulting in an early pubertal onset and an early decline in reproductive lifespan.
孕期限制饮食会影响后代成年后的健康。本研究旨在观察母体饮食限制（MFR）对第一代（FR1）和第二代（FR2）雌性大鼠后代生殖性能的影响。
成年处女 Wistar 雌性大鼠自由饮用自来水，并自由摄取标准啮齿动物饲料，与成年雄性大鼠交配，然后随机分为两组：对照组（自由摄取）和饮食限制组（FR，整个妊娠期仅摄取 50%的自由摄取食物）。它们的第一代（FR1）和第二代（FR2）后代自由摄取食物，并在青春期前和成年期前处死。取出它们的卵巢，通过估计卵泡数量（总卵泡数和各个卵泡发育阶段的卵泡数）以及多核卵母细胞和多卵母细胞卵泡的存在来评估其组织学。
与对照组相比，FR1 雌性在第 4 周时卵巢总卵泡数较低，而 FR2 雌性则无差异。与对照组相比，FR1 和 FR2 雌性在第 4 周和第 8 周时原始卵泡数均较低。与对照组相比，FR1 和 FR2 雌性在第 4 周时含有多核卵母细胞的卵泡更为频繁，而在第 8 周时则分别较高和较低。
MFR 通过在第一代和第二代后代的卵巢中诱导异常卵泡的发育来影响卵巢组织学。这一发现可能会影响卵巢功能，导致青春期提前和生殖寿命早期下降。
