Zheng Pei-Pei, Zhang Li-Na, Zhang Jie, Chang Xin-Miao, Ding Shan, Xiao Fei, Guo Li-Xin
Department of Endocrinology, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, People's Republic of China.
Peking University Fifth School of Clinical Medicine, Beijing 100730, People's Republic of China.
Nat Sci Sleep. 2020 Nov 20;12:1053-1065. doi: 10.2147/NSS.S271318. eCollection 2020.
To study the effects of chronic-simulated night shift work using the rat model and examines if a particular sleep supplement mode could be better in alleviating the effects.
The male Wistar rats were randomly divided into the control (CTL: 8 rats) and night shift work (NW: 24 rats) groups of rats. Based on the sleep supplement strategy, the NW group was further segregated into three subgroups (8 rats each); late sleep supplement group (LSS), early sleep supplement group (ESS), and intermittent sleep supplement group (ISS). Sleep deprivation was achieved using the standard small-platform-over water method. Parameters such as animal body weight and food intake were measured daily. The intraperitoneal glucose tolerance test, fasting plasma insulin concentration, insulin resistance index and insulin sensitivity were measured twice, in the 4th and 8th weeks of the study. Plasma corticosterone concentration and pathological changes in islets (insulitis) were measured at the end of the 8th week.
In NW group, night work resulted in a gain of body weight and albeit lower than that of the CTL group. NW rats also had higher food intake, showed impaired glucose metabolism and higher plasma corticosterone concentration. The sleep supplement experiments suggested that compared to the other modes, intermittent sleep supplement had significantly low changes in the body weight, glucose metabolism and the islet cells.
Similar to previous studies, we also found that night shift work adversely impacts the body weight and glucose metabolism in rats. However, upon evaluating different sleep supplement strategies, we found the intermittent sleep supplement strategy to be most effective.
利用大鼠模型研究慢性模拟夜班工作的影响，并探讨特定的睡眠补充模式是否能更好地减轻这些影响。
将雄性Wistar大鼠随机分为对照组（CTL：8只大鼠）和夜班工作组（NW：24只大鼠）。根据睡眠补充策略，NW组进一步分为三个亚组（每组8只）；晚睡补充组（LSS）、早睡补充组（ESS）和间歇睡眠补充组（ISS）。采用标准的小平台悬水法实现睡眠剥夺。每天测量动物体重和食物摄入量等参数。在研究的第4周和第8周，两次测量腹腔葡萄糖耐量试验、空腹血浆胰岛素浓度、胰岛素抵抗指数和胰岛素敏感性。在第8周结束时测量血浆皮质酮浓度和胰岛病理变化（胰岛炎）。
在NW组中，夜班工作导致体重增加，尽管低于CTL组。NW组大鼠的食物摄入量也更高，葡萄糖代谢受损，血浆皮质酮浓度更高。睡眠补充实验表明，与其他模式相比，间歇睡眠补充在体重、葡萄糖代谢和胰岛细胞方面的变化显著较低。
与先前的研究类似，我们还发现夜班工作对大鼠的体重和葡萄糖代谢有不利影响。然而，在评估不同的睡眠补充策略后，我们发现间歇睡眠补充策略最为有效。
