Mohebbi Seyed Reza, Baghaei Kaveh, Rostami-Nejad Mohammad, Nazemalhosseini Mojarad Ehsan, Mirjalali Hamed, Yadegar Abbas, Asri Nastaran, Abdoulahi Shahrokh, Assadzadeh Aghdaei Hamid
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterol Hepatol Bed Bench. 2020 Fall;13(4):388-392.
Evaluating the expression level of CD4 FoxP3 CD25 T cells and IL-6 in peripheral blood samples of hospitalized COVID-19 patients.
COVID-19 is an emerging disease with worldwide distribution. However, there is a little data about the correlation between the disease and the host immune responses.
Whole blood samples of 30 COVID-19 patients and eight healthy people were collected during March to June 2020. Total RNA was extracted from the samples, cDNA synthesis was performed, and the expression level of targeted genes was evaluated using quantitative real-time PCR.
The expression level of CD4, CD25, and Foxp3 was significantly downregulated 5-, 2-, and 3-fold, respectively, among COVID-19 patients in comparison to healthy controls (-value < 0.0001). The expression level of IL-6 represented almost 18-fold increase in COVID-19 patients compared to healthy controls.
Our findings indicated the expression profile analysis of CD4 FoxP3 CD25 T cells could be a potential marker for the assessment of severity of COVID-19 patients.
评估住院的新型冠状病毒肺炎（COVID-19）患者外周血样本中CD4⁺FoxP3⁺CD25⁺T细胞和白细胞介素-6（IL-6）的表达水平。
COVID-19是一种在全球范围内传播的新兴疾病。然而，关于该疾病与宿主免疫反应之间相关性的数据较少。
于2020年3月至6月收集30例COVID-19患者和8名健康人的全血样本。从样本中提取总RNA，进行cDNA合成，并使用定量实时聚合酶链反应评估靶向基因的表达水平。
与健康对照相比，COVID-19患者中CD4⁺、CD25⁺和Foxp3⁺的表达水平分别显著下调了5倍、2倍和3倍（P值<0.0001）。与健康对照相比，COVID-19患者中IL-6的表达水平增加了近18倍。
我们的研究结果表明，CD4⁺FoxP3⁺CD25⁺T细胞的表达谱分析可能是评估COVID-19患者病情严重程度的潜在标志物。
